Quantification of leukocyte dynamics in bone marrow of healthy humans

Recruiting

• Conditions: immunological memory; maintenance of immune cells; 10047954

• Registration Number: NL-OMON56359
• Lead Sponsor: niversitair Medisch Centrum Utrecht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 20

Inclusion Criteria

Elective hip or knee replacement surgery, older than 55 years of age

Exclusion Criteria

(Pathological) fracture as surgery indication, chronic infection, daily use of
immuno-modulatory drugs

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The average turnover of different immune cell subsets in bone marrow of healthy<br /><br>human adults during homeostasis.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- The average turnover and lifespan of antigen-specific T cell subsets in bone<br /><br>marrow and paired blood;<br /><br>- The phenotype of immune cells in bone marrow and paired blood based on flow<br /><br>cytometric markers (percentage positive and mean fluorescence intensity).<br /><br>- The function of immune cells in bone marrow and paired blood based on in<br /><br>vitro stimulation assays, e.g. in IFN-g or TNF-a production.<br /><br>- Concentration of factors in plasma and bone marrow and paired blood that can<br /><br>influence leukocyte dynamics, such as growth and survival factors, infection<br /><br>parameters, and viral factors.<br /><br>- Comparison of the T-cell receptor (TCR) repertoire between blood and bone<br /><br>marrow;<br /><br>- Determination of the phenotype of adaptive NK cells in blood and bone marrow<br /><br>using scATAC-seq.</p><br>