The Effect of Cryostimulation on Inflammation and Motor Functions. The Study Was Conducted to Determine the Effect of Five Days of Whole-body Cryostimulation (PBC) on Reducing Inflammation and Improving Motor Skills in Soccer Players

Not Applicable

Completed

• Conditions: Training; Rest
• Interventions: Other: Criostymulation

• Registration Number: NCT06549933
• Lead Sponsor: Poznan University of Physical Education

Brief Summary

The aim of the study was to determine the effect of a 5-day PBC on reducing inflammation and improving motor skills in soccer players during the initial phase of the training period.

The five-day cryostimulation applied did not significantly affect the profile of inflammatory markers in soccer players. However, a reduction in visuo-motor reaction time (RT) was registered in PBC-treated soccer players, suggesting the potential benefits of this method in improving motor skills.

Detailed Description

Cryostimulation is one of the methods of post-exercise recovery, involving short-term (3 min) exposure of the body to extremely low temperatures, oscillating between -100°C and -190°C. The purpose of this therapy is to reduce inflammation, reduce physical and mental fatigue and use it as a passive form of preparing the body for further exercise as part of the training process.

The study was conducted to determine the effect of five days of whole-body cryostimulation (PBC) on reducing inflammation and improving motor skills in soccer players. A group of 24 football players was randomly divided into a test group (PBC; -140°C ± 20°C, 3 min, 5 days) and a control group (CON). Before the cryostimulation session, both groups were subjected to visual-motor ability tests, which were repeated on the last day of the study. Blood samples were collected at four time points (P0-1 day, P1-3 day, P2-5 day, P3-2 days after the test). Levels of creatine kinase (CK), tumor necrosis factor (TNFα), interleukins (IL-6, IL-10), testosterone (T) and cortisol (C) were determined in the samples.

Study Timeline

• Study Start: 2023-11-25
• Primary Completion: 2024-02-24
• Study Completion: 2024-04-15
• Status Verified: 2024-07
• Study First Submitted: 2024-07-24
• Study First Submitted QC: 2024-08-08
• Study First Posted: 2024-08-12
• Last Update Submitted: 2025-01-15
• Last Update Posted: 2025-01-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 21

Inclusion Criteria

• athletes
• no previous experience with systemic cryotherapy
• any other forms of recovery or cosmetic treatments

Exclusion Criteria

• alcohol or any other stimulants
• anti-inflammation drugs
• injuries

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Cryostimulation	Criostymulation	Cryostimulation performed in a cryosauna for five consecutive days.

Primary Outcome Measures

Name	Time	Method
CK (creatine kinase)	Before, after 3, and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit
TNF-alpha (Tumor necrosis factor-alpha)	Before, after 3 days and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit
Cortisol	Before, after 3, and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit
IL-6 (Interleukin-6)	Before, after 3 days and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit
Testosterone	Before, after 3, and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit
IL-10 (Interleukin-10)	Before, after 3, and 5 days of cryostimulation	Immunoenzymatic assay method using a diagnostic ELISA Kit

Secondary Outcome Measures

Name	Time	Method
Anthropometric measurments	Before, after 3, and 5 days of cryostimulation	Analysis of body composition, Tanita BC-418

Trial Locations

Locations (1)

Poznań University of Physical Education; 🇵🇱 Poznań, Poland