The Acute Effects of an Endurance Exercise Bout the Gut Microbiome and Gut-derived Metabolome in Women's Distinct Fitness and Body Mass Indexes
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Gut Microbiome Composition
研究概览
简要总结
This study aims to elucidate the differences in the gut microbiome functional activity and metabolome in adult premenopausal women with distinctive fitness levels and BMIs (with obesity, w/o obesity). The specific aims are as follows:
- Aim 1: To examine the effects of acute aerobic exercise at 60-70% heart rate reserve (HRRmax) for 30 minutes bout on changes in the abundance of SCFA-producing bacteria and their functional downstream metabolic activity.
- Aim 2: To examine the effects of acute aerobic exercise at 60-70% HRRmax 30-minute bout on changes in GM-released SCFA concentrations in stool and plasmatic metabolome.
详细描述
Participants who are eligible after completing the online questionnaire will be invited to three in-person visits:
VISIT A (baseline visit)
- Participants will receive oral and written information about the study and be allowed to ask questions.
- Participants will sign the informed consent document.
- Inclusion and exclusion criteria will be reviewed.
- Demographic, health, and lifestyle data will be collected.
- Collection of height and weight.
- Body composition analysis via DXA scan
- Strength test: isometric mid-thigh pull and hand-grip strength test.
- Endurance test: A cardiovascular fitness test using a cycle ergometer will be performed.
- Participants will be provided with a hip accelerometer and at-home stool sample collection kit, and an account will be set up using Cronometer software to track their dietary intake during the study.
At VISIT B (exercise test day), after 7 to 12 days after VISIT A, the following procedures will happen:
- Participants will return a stool sample collected within the past 5 days.
- 7 mL of blood draw
- Performed a 30-minute biking exercise bout at their individually calculated 60 to 70% of the HRR.
- 7 mL of blood draw 3 minutes after the exercise
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 21 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Biological sex: female
- •BMI 19 to 24.99 kg/m2 or BMI 30-40 kg/m2
- •Exercisers: Exercise at least 4 times per week for 60 minutes (a total of 240 minutes per week) on a programmed exercise training either on endurance sports (e.g., running, cycling, triathlon), strength (e.g., powerlifting), or team sports (e.g., rugby, football, soccer)
- •Non-Exercisers: Sedentary individuals who have not reached the PA guidelines (150 minutes of moderate-intensity physical activity a week, 75 minutes of vigorous-intensity physical activity, or an equivalent combination of moderate and vigorous-intensity physical activity).
排除标准
- •Biological sex: male
- •BMI < 19 kg/m2 or > 40 kg/m2
- •Currently pregnant, < 2 years postpartum, lactating
- •Currently taking any herbal, fiber, or prebiotic supplement
- •Current or 1-month before the study, taking oral or vaginal antibiotics
- •Diagnosed with any gastrointestinal, endocrine, digestive, cancer, or cardiovascular disease.
研究组 & 干预措施
Intervention
30-minute exercise bout in a cycle ergometer at 60 to 70% of the Heart Rate Reserve (HRR) and corresponding power (watts).
干预措施: Aerobic Exercise (Behavioral)
结局指标
主要结局
Gut Microbiome Composition
时间窗: Before the exercise intervention and immediately after the intervention
Shotgun metagenomics will be used to examine the abundance of specific SCFA-producing bacteria in the stool (e.g., Ruminococcus)
Gut Microbiome Function
时间窗: Before the exercise intervention and immediately after the intervention
Shotgun metagenomics will be used to examine the BGCs (i.e., the genes within the microbial genome that dictate gut microbial functionality) to investigate functional downstream metabolic activity.
Plasma Metabolome
时间窗: Before the exercise intervention, immediately after the exercise intervention, and 24 hours before the intervention
Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in plasma before and after the exercise intervention
Stool Metabolome
时间窗: Before the exercise intervention, immediately after the exercise intervention, and 24 hours before the intervention
Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in stool before and after the exercise intervention
次要结局
未报告次要终点