Evidence of a Redox-dependent Regulation of Immune Responses to Exercise-Induced Inflammation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Changes in protein carbonyls in red blood cells
研究概览
简要总结
In this investigation the investigators utilized N-acetylcysteine (NAC) supplementation to enhance reduced glutathione (GSH) stores during an 8-day recovery period from a strenuous eccentric exercise protocol in order to test the hypotheses: i) redox status perturbations in skeletal muscle are pivotal for the immune responses and ii) antioxidant supplementation may alter immune cell responses following exercise-induced muscle microtrauma.
详细描述
The major thiol-disulfide couple of GSH and oxidized glutathione (GSSG) is a crucial regulator of the main transcriptional pathways regulating aseptic inflammation and recovery of skeletal muscle following aseptic injury. Antioxidant supplementation may hamper exercise-induced inflammatory responses.
The objective was to examine how thiol-based antioxidant supplementation affects immune mobilization following exercise-induced skeletal muscle microtrauma. In a two-trial, double-blind, crossover, repeated measures design, 10 young men received either placebo or NAC (20 mg/kg/day) immediately after a muscle-damaging exercise protocol (300 eccentric contractions) and for eight consecutive days. Blood sampling and performance assessment were performed pre-exercise, 2h post-exercise and daily for 8 consecutive days.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Recreationally trained (VO2max > 45 ml/kg/min)
- •Engaged in regular exercise for ≥3 times/week for > 12 months
- •non-smokers
- •Abstain from exercise during the course of the two trials
- •No consumption of performance-enhancing substances, antioxidants, caffeine, alcohol and/or medications during the study.
排除标准
- •NAC intolerance
- •Recent musculoskeletal injuries of the lower limbs
- •Febrile illness
- •History of muscle lesion.
结局指标
主要结局
Changes in protein carbonyls in red blood cells
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Concentration of protein carbonyls
Changes in total antioxidant capacity in serum
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Changes in oxidized glutathione in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Concentration of oxidized glutathione in red blood cells
Changes in creatine kinase activity in serum
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Changes in lymphocyte count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of lymphocyte count in blood
Changes in thiobarbituric acid reactive substances in red blood cells
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Thiobarbituric acid reactive substances concentration in red blood cells
Changes in reduced glutathione in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Concentration of reduced glutathione in red blood cells
Changes in high sensitivity C-reactive protein in serum
时间窗: Pre-exercise, 2 hours post-exercise, 1 day post-exercise, 2 days post-exercise, 3 days post-exercise
Changes in catalase activity in red blood cells
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Changes in natural killer (NK) cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of natural killer cell count in blood
Changes in HLA+/Macr+ macrophage count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of HLA+/Macr+ count in blood
Changes in adhesion molecule concentration in blood
时间窗: Pre-exercise, 2 hours post-exercise, 1 day post-exercise, 2 days post-exercise, 3 days post-exercise
Measurement of soluble vascular cell adhesion molecule-1 (sVCAM-1) and soluble intercellular cell adhesion molecule-1 (sICAM-1) concentrations in plasma
Changes in neutrophil count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of neutrophil count in blood
Changes in basophil count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of baseophil count in blood
Changes in eosinophil count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of eosinophil count in blood
Changes in T cytotoxic cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of T cytotoxic cell count in blood
Changes in white blood cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Changes in cytokine concentration in serum
时间窗: Pre-exercise, 2 hours post-exercise, 1 day post-exercise, 2 days post-exercise, 3 days post-exercise, 8 days post-exercise
Measurement of interleukin-1β (IL-1β) and interleukin-6 (IL-6)
Changes in T-helper cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of T-helper cell count in blood
Changes in natural killer-T (NK-T) cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of NK-T cell count in blood
Changes in monocyte count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of monocyte count in blood
Changes in macrophage count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of macrophage count in blood
Changes in B lympho cell count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of B lympho cell count in blood
Changes in 62L macrophage count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of 62L macrophage count in blood
Changes in 11B+ macrophage count in blood
时间窗: Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise
Cytofluorometric analysis of 11B+ macrophage count in blood
次要结局
- Changes in muscle performance(Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise)
- Changes in delayed onset of muscle soreness(Pre-exercise, 2 hours post-exercise, daily for 8 consecutive days post-exercise)
- Maximal aerobic capacity(One day before exercise)
- Body composition(One day before exercise)
- Changes in dietary intake profile(One day before exercise and daily for 8 consecutive days post-exercise)
研究者
Alexandra Sakelliou
PhD candidate in Department of Diabetes and Metabolism Unit
National and Kapodistrian University of Athens