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One Week of Magnesium Supplementation Lowers IL-6, Perceived Pain and Increases Post Exercise Blood Glucose in Response to Downhill Running

Not Applicable
Completed
Conditions
Magnesium Deficiency
Interventions
Dietary Supplement: Magnesium oxide
Dietary Supplement: Placebo
Registration Number
NCT04216836
Lead Sponsor
University of Worcester
Brief Summary

This study investigated the effect of magnesium supplementation on exercise performance and functional recovery in recreational endurance athletes in conjunction with measures of blood glucose, lactate, IL-6 and sIL-6R.

Detailed Description

Magnesium status can directly affect circulating glucose concentrations both during and post exercise. In addition, magnesium supplementation has been shown to reduce circulating IL-6 concentrations post exercise in humans. It is conceivable that such observations are linked through the role of IL-6 in glucose regulation, possibly in combination with sIL-6R. Together, magnesium intake may have the potential to effect exercise performance and recovery through glucose availability. This in turn may be connected to the production of IL-6 and sIL-6R which have been established to influence exercise fatigue and perception of pain (muscle soreness).

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
9
Inclusion Criteria
  • Regular recreational runner, running around 3 times per week
  • Capable of running 10 km in ~ 40 minutes.
Exclusion Criteria
  • Any signs or symptoms of cardiovascular issues.
  • Any recent form of injury or illness.
  • Currently, or in the last 3 months, have consumed multivitamin supplements
  • Currently, or in the last 3 months, have consumed anti-inflammatory medications.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
High magnesium diet (SUP condition)Magnesium oxideParticipants followed a low magnesium diet \<260mg/day and consumed 500 mg/day of magnesium oxide. This was separated into 3 capsules, which were consumed at 6 hr intervals each day (8am, 2pm and 8pm). The supplementation period was 1 week.
Low magnesium diet (CON condition)PlaceboParticipants followed a low magnesium diet \<260mg/day and consumed 500 mg/day of placebo (cornflour). This was separated into 3 capsules, which were consumed at 6 hr intervals each day (8am, 2pm and 8pm). The supplementation period was 1 week.
Primary Outcome Measures
NameTimeMethod
24 hr post exercise maximal force testing of the dominant leg on the isokinetic dynamometer1 day

Maximal force produced from the dominant leg (eccentric and concentric) on an isokinetic dynamometer.

Downhill 10 km treadmill time trial performance1 day

Maximal 10 km time trial performance on a treadmill

Secondary Outcome Measures
NameTimeMethod
Interleukin-6up to 2 days

Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. Enzyme-linked immunosorbent assays were used to analyse interleukin-6.

Lactateup to 2 days

Capillary blood samples at rest, during, immediately post, 1 hr post and 24 hrs post 10 km downhill time-trial. A Biosen analyser was used to analyse lactate concentrations.

Glucoseup to 2 days

Capillary blood samples at rest, during, immediately post, 1 hr post and 24 hrs post 10 km downhill time-trial. A Biosen analyser was used to analyse glucose concentrations.

Perceived muscle sorenessup to 4 days

A 10cm visual analogue scale was used to assess perceived muscle soreness, the scale started at 0 (no pain) and finished at 10 (unbearable pain)

Creatine kinaseup to 2 days

Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. A Reflotron analyser was used to analyse creatine kinase concentrations.

Soluble interleukin-6 receptorup to 2 days

Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. Enzyme-linked immunosorbent assays were used to analyse soluble interleukin-6.

Trial Locations

Locations (1)

University of Worcester

🇬🇧

Worcester, Worcestershire, United Kingdom

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