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Safety and Efficacy of TUDCA as add-on Treatment in Patients Affected by ALS

Phase 3
Active, not recruiting
Conditions
Amyotrophic Lateral Sclerosis (ALS)
Interventions
Registration Number
NCT03800524
Lead Sponsor
Humanitas Mirasole SpA
Brief Summary

This is a Phase III, multi-centre, randomized, double-blind, placebo-controlled, parallel-group study to evaluate Safety and Efficacy of Tauroursodeoxycholic (TUDCA) as add-on Treatment in Patients Affected by Amyotrophic Lateral Sclerosis (ALS).

Detailed Description

Enrolled patients will be randomized to one of two treatment arms: TUDCA or identical placebo by oral route. The randomization will be performed in a ratio one to one for the two arms.

TUDCA will be administered orally at the dose of 1 g twice daily (2 g daily) for 18 months. Patients will be taking also riluzole at the dose of 50 mg twice daily (100 mg daily).

Patient randomization will take place after a screening (lead-in) period of 12 weeks (3 months) with 3 assessments at 6-week intervals. Clinical assessments during the trial phase will be performed every three months. This will allow measuring the progression rate before and after starting treatment (either active or placebo).

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
337
Inclusion Criteria
  • Probable laboratory-supported, probable, or definite ALS, as defined by El Escorial Revised ALS diagnostic criteria at screening visit (month -3)
  • Disease duration ≤ 18 months at screening visit (month -3)
  • Able to perform reproducible pulmonary function tests at screening visit (month -3)
  • Forced vital capacity or slow vital capacity ≥70% of normal at screening visit (month -3)
  • Stable on riluzole treatment for 3 months in the lead-in period
  • Signed informed consent at screening visit (month -3)
Exclusion Criteria
  • Treatment with edaravone
  • Other causes of neuromuscular weakness
  • Presence of other neurodegenerative diseases
  • Significant cognitive impairment, clinical dementia or psychiatric illness
  • Severe cardiac or pulmonary disease
  • Other diseases precluding functional assessments
  • Other life-threatening diseases
  • Any use of non-invasive ventilation (e.g. continuous positive airway pressure, non-invasive bi-level positive airway pressure or non-invasive volume ventilation) for any portion of the day, or mechanical ventilation via tracheostomy, or on any form of oxygen supplementation
  • Gastrointestinal disorder that is likely to impair absorption of study drug from the gastrointestinal tract
  • Has taken any investigational study drug within 30 days or five half-lives of the prior agent, whichever is longer, prior to dosing
  • Any clinically significant laboratory abnormality
  • Other concurrent investigational medications
  • Active peptic ulcer
  • Previous surgery or infections of small intestine
  • Patients unable to easily swallow the treatment pills
  • Acute inflammation of the gallbladder or bile ducts
  • Occurrence of frequent biliary colic, biliary infections, severe pancreatic abnormalities
  • Bile duct obstruction, calcified X-ray opaque gallstones and reduced mobility of the gallbladder
  • Subjects who weigh 88 lbs (40 kg) or less
  • Aspartate aminotransferase or alanine aminotransferase concentrations more than 3 times the upper limit of normal
  • Creatinine clearance 50 ml/min or less
  • Any clinically significant neurological, haematological, autoimmune, endocrine, cardiovascular, neoplastic, renal, gastrointestinal, or other disorder that, in the Investigator's opinion, could interfere with the subject's participation in the study, place the subject at increased risk, or confound interpretation of study results
  • Consideration by the investigator, for any reason, that the subject is an unsuitable candidate to receive TUDCA or that the subject is unable or unlikely to comply with the dosing schedule or study evaluations
  • The patient of reproductive potential is sexually active and is not willing to use highly effective contraception during the study and up to 90 days after the day of last dose
  • The patient is pregnant or breast feeding

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Tauroursodeoxycholic acid (TUDCA)Tauroursodeoxycholic Acid* Tauroursodeoxycholic acid (TUDCA) 250 mg capsules * Doses: 4 capsules (1 g) twice daily 10-15 minutes after a meal * Mode of administration: orally * Duration: 18 months
Reference therapyPlacebo* Placebo capsules identical to active compound * Doses: 4 capsules (1 g) twice daily 10-15 minutes after a meal * Mode of administration: orally * Duration: 18 months
Primary Outcome Measures
NameTimeMethod
Number of responder patients18 months

Identification of the responder patients defined as those showing an improvement of at least 20% in the ALSFRS-R slope

Secondary Outcome Measures
NameTimeMethod
MMP-9 levels18 months

Effect of TUDCA on MMP-9 expression in comparison to placebo

Medical Research Council (MRC) scale18 months

Difference in change from baseline in muscle force assessed by the MRC scale. The scale rates muscle strength of 6 muscles (3 at the upper and 3 at the lower limbs), bilaterally. Each muscle is graded from 0 = no movement, to 5 = normal strength, giving a total sum-score that ranges from 0 (total paralysis) to 60 (normal strength).

Neurofilaments levels18 months

Effect of TUDCA on Neurofilament levels in comparison to placebo

Survival time18 months

Survival time measured by death or respiratory insufficiency

ALS disease functional rating scale - revised version (ALSFRS-R)18 months

Difference in change from baseline in ALSFRS-R. Each task of the scale is rated on a five-point scale from 0 = can't do, to 4 = normal ability. Individual item scores are summed to produce a reported score ranging from 0 = worst to 48 = best.

ALS Assessment Questionnaire-40 (ALSAQ-40)18 months

Difference in change from baseline in ALSAQ-40. The instrument contains 40 statements that measure five dimensions of health state: Physical Mobility (10 statements), Activities of Daily Living and Independence (10 statements), Eating and Drinking (5 statements), Communication (5 statements), Emotional Functioning (10 statements). The patient must indicate how often (Never, Rarely, Sometimes, Often, or Always) the statement have been true. Dimension scores are coded on a likert scale, ranging from 0 (best health as assessed by the scale) to 100 (worse health as assessed by the measure).

Forced Vital Capacity18 months

Difference in change from baseline in Forced Vital Capacity

EuroQol 5-Dimension-5 Levels (EQ-5D-5L) scale18 months

Difference in change from baseline in EQ-5D-5L scale. The EQ-5D-5L descriptive system comprises 5 dimensions (mobility, self-care, usual activities, pain/discomfort and anxiety/depression). Each dimension has 5 levels: 1.no problems, 2.slight problems, 3.moderate problems, 4.severe problems, 5.extreme problems. The patient is asked to indicate his/her health state by ticking (or placing a cross) in the box against the most appropriate statement in each of the 5 dimensions. This decision results in a 1-digit number expressing the level selected for that dimension. The digits for 5 dimensions can be combined in a 5-digit number describing the patient health state. Numbers 1-5 have no arithmetic properties and should not be used as a cardinal score.

Long-term safety and tolerability18 months

assessed through adverse reaction, concomitant treatment, physical examination, vital signs and routine haematology and biochemistry analyses

Trial Locations

Locations (25)

Alfried Krupp Krankenhaus Rüttenscheid

🇩🇪

Essen, Germany

Medizinische Hochschule Hannover

🇩🇪

Hannover, Germany

The Walton Centre NHS Foundation Trust

🇬🇧

Liverpool, United Kingdom

Centre Hospitalier Universitaire de Bordeaux

🇫🇷

Bordeaux, France

IRCCS Istituto Auxologico Italiano

🇮🇹

Milano, Italy

Royal Stoke University Hospital

🇬🇧

Stoke, United Kingdom

AOU Città della Salute e della Scienza di Torino

🇮🇹

Torino, Italy

Lancashire Teaching Hospitals NHS Foundation Trust

🇬🇧

Preston, United Kingdom

Salford Royal NHS Foundation Trust

🇬🇧

Salford, United Kingdom

University of Sheffield

🇬🇧

Sheffield, United Kingdom

Plymouth Hospitals NHS Trust

🇬🇧

Plymouth, United Kingdom

Trinity College Dublin

🇮🇪

Dublin, Ireland

Katholieke Universiteit Leuven

🇧🇪

Leuven, Belgium

IRCCS Istituto Clinico Humanitas

🇮🇹

Rozzano, Italy

NEuroMuscular Omnicentre. Fondazione Serena Onlus

🇮🇹

Milano, Italy

Azienda Ospedaliera Santa Maria di Terni

🇮🇹

Terni, Italy

AOU Università degli Studi della Campania "Luigi Vanvitelli"

🇮🇹

Napoli, Italy

Centre Hospitalier Universitaire Limoges

🇫🇷

Limoges, France

Centre Hospitalier Universitaire de Montpellier

🇫🇷

Montpellier, France

Universitätsklinikum Carl Gustav Carus Dresden

🇩🇪

Dresden, Germany

Centre Hospitalier Regional Universitaire de Tours

🇫🇷

Tours, France

Charité - Universitätsmedizin Berlin

🇩🇪

Berlin, Germany

Universitätsklinikum Jena

🇩🇪

Jena, Germany

Universität Ulm

🇩🇪

Ulm, Germany

Universitair Medisch Centrum Utrecht

🇳🇱

Utrecht, Netherlands

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