Lemon Verbena Extract Oxidative Stress and Muscle Damage

Not Applicable

Completed

• Conditions: Muscle Injury; Muscle Soreness
• Interventions: Dietary Supplement: maltodextrin; Dietary Supplement: Lvemon verbena extract

• Registration Number: NCT04742244
• Lead Sponsor: National Taiwan Sport University

Brief Summary

In the present study, investigators investigated whether supplementation with lemon verbena extract (LVE) could improve muscle damage and biochemical indicators after exhaustive exercise challenge. Based on maximum jump heights, 60 subjects (30 males and 30 females) were equally divided into a placebo group (0 mg/human/day) and an LVE supplement group (400 mg/human/day), with gender-equal distribution. All subjects started supplementation 10 days before exhaustive exercise and continued it until all tests were completed. Before the intervention, after the exhaustive exercise, and on the following 3 days, the participants underwent 12-minute Cooper running/walking; blood collection; assessments of pain, muscle stiffness, maximum jump heights, and isometric maximum muscle strength; and anaerobic strength tests.

Detailed Description

Not available

Study Timeline

• Study Start: 2020-05-28
• Primary Completion: 2020-07-28
• Study Completion: 2020-08-13
• Study First Submitted: 2021-01-25
• Study First Submitted QC: 2021-02-02
• Study First Posted: 2021-02-08
• Status Verified: 2021-03
• Last Update Submitted: 2021-03-08
• Last Update Posted: 2021-03-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

• health
• Must be able to swallow tablets

Exclusion Criteria

• non-smokers
• no musculoskeletal
• medical
• metabolic disease
• women not on the reproductive cycle
• high blood pressure, asthma
• skeletal neuromuscular injuries in the upper or lower extremities

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo	maltodextrin	0 mg LVE/capsule, 2capsule/day, 14days
Lemon Verbena extract	Lvemon verbena extract	200 mg LVE/capsule, 2capsule/day, 14days

Primary Outcome Measures

Name	Time	Method
Clinical Biochemistry of CK level	10 days	To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan).
Assessment of muscle stiffness	10 days	3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia)
inflammation of IL-6 level	10 days	To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R\&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis
Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine	10 days	Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis
Visual Analogue Scale (VAS) evaluation	10 days	3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain

Trial Locations

Locations (1)

National Taiwan Sport University; 🇨🇳 Taoyuan, Taiwan