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Gluten-free Diet Monitoring in Urine

Early Phase 1
Completed
Conditions
Celiac Disease
Registration Number
NCT02344758
Lead Sponsor
University of Seville
Brief Summary

The purpose of this study is to develope a novel method to determine gluten intake and to check gluten-free diet adherence in celiac patients by detection of gluten immunogenic peptides in urine.

Detailed Description

To date, the mainstay of the management of CD is a strict life-long adherence to gluten-free diet (GFD). Follow-up monitoring of the improvement of patients on GFD is essential to assess symptomatic recovery and to monitor complications, as well as to assist patients with dietary compliance. However, practical methods to monitor diet compliance and to detect the origin of an outbreak of celiac clinical symptoms are not available.

In this study, we have overcome these challenges and shown the feasibility of measuring gluten immunogenic peptides (GIP) in urine samples in healthy and celiac individuals by solid phase extraction and estimating the peptide concentrations with a reader of anti-GIP moAb immunochromatographic strips (IC-strips).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
134
Inclusion Criteria
  • Celiac patient: childs (< 16 years) and adults (> 16 years)
  • written informed consent
Exclusion Criteria
  • presence of family history of CD
  • digestive disease symptoms
  • known medical disease
  • use of prescription medications and use of antibiotics and probiotics in the previous 2 months to the inclusion in the study
  • participation in any other studies involving investigational concomitantly or within two weeks prior to entry into the study and during the course of the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Primary Outcome Measures
NameTimeMethod
Usual gluten-containing diet for healthy individuals1 month
Secondary Outcome Measures
NameTimeMethod
Usual gluten-free diet for celiac patients (home diet not modified for this trial)1 month

Trial Locations

Locations (1)

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville

🇪🇸

Seville, Spain

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville
🇪🇸Seville, Spain

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