Comparative analysis of Odonto/Osteogenic Potential of human gingival mesenchymal stem cells in 2D and 3D culture systems
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 5
- 试验地点
- 1
- 主要终点
- The present study compares odontogenic and osteogenic differentiation potential of human gingival mesenchymal stem cells when cultured under 2d and 3d culture systems.
研究概览
简要总结
Periodontitis is a chronic inflammatory condition leading to destruction of tooth-supporting tissues and eventual tooth loss. Regenerative therapy aims to restore lost periodontal structures, and stem cell–based approaches have gained prominence due to their self-renewal and multilineage differentiation potential. Among dental stem cells, human gingival mesenchymal stem cells are easily accessible, highly proliferative, and exhibit strong osteogenic potential.
The present study aims to compare the odonto-osteogenic differentiation potential of GMSCs cultured under two-dimensional 2D and three-dimensional 3D systems. Healthy gingival tissues will be collected from patients undergoing routine dental procedures. Isolated GMSCs will be characterized for MSC surface markers CD73, CD90, CD105 and induced toward osteogenic differentiation under both 2D and 3D conditions. The 3D cultures will be established using the hanging drop spheroid method.
Comparative evaluation will be performed through morphological assessment, cytochemical staining ALP, Alizarin Red S, and molecular analysis of osteogenic markers by RT-PCR. It is hypothesized that 3D-cultured GMSCs will demonstrate enhanced osteogenic differentiation due to improved cell–cell and cell–matrix interactions that better mimic in vivo conditions.
The study is expected to provide valuable insight into optimizing culture conditions for GMSC-based regenerative applications in periodontal and alveolar bone tissue engineering
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 30.00 Year(s)（—）
- 性别
- All
入选标准
- •1.Systemically healthy patients with a minimum complement of 20 natural teeth undergoing extraction of premolars for orthodontic treatment.
- •2.Patients with good oral hygiene (Plaque index less than 1).
- •3.Periodontally healthy patients (less than 1 Gingival index score, less than 3 mm PD and CAL less than 2 mm).
- •4.Age group between 18 and 30 years.
排除标准
- •1.Patients with history of any systemic disease.
- •2.Patients on antibiotic therapy of any form for the last 6 months.
- •3.Presence of any periodontal disease.
- •4.History of any form of tobacco usage.
- •5.Pregnant or lactating women.
结局指标
主要结局
The present study compares odontogenic and osteogenic differentiation potential of human gingival mesenchymal stem cells when cultured under 2d and 3d culture systems.
时间窗: 4 weeks to 8 weeks
The finding of this study will contribute to better understanding of regenerative potential of gingival stem cells and aid in developing scaffold based strategies for periodontal regeneration
时间窗: 4 weeks to 8 weeks
次要结局
未报告次要终点
研究者
Srilakshmi
NITTE (Deemed to be University)