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A Study of TBio-4101 (TIL) and Pembrolizumab in Patients With Advanced Solid Tumors

Phase 1
Terminated
Conditions
Breast Cancer
Colorectal Cancer
Cutaneous Melanoma
Uveal Melanoma
Non-Small Cell Lung Cancer
Head and Neck Squamous Cell Carcinoma
Interventions
Registration Number
NCT05576077
Lead Sponsor
Turnstone Biologics, Corp.
Brief Summary

A multicenter trial to investigate TBio-4101, an autologous, neoantigen-selected, tumor-reactive TIL product, in patients with advanced solid malignancies.

Detailed Description

This is a Phase 1 study to investigate TBio-4101. TBio-4101 is an autologous tumor infiltrating lymphocyte (TIL) therapy that utilizes tumor specific antigens to select, sort, and expand patient-specific tumor-reactive T-cells to be reinfused into the patient. The adoptive cell therapy is further enhanced through the use of non-myeloablative chemotherapy prior to TIL infusion, followed by the TIL plus IL-2 infusion. Low-dose radiation therapy is administered prior to and after TIL plus IL-2 infusion. Pembrolizumab is provided after the resolution of IL-2 toxicities. The trial is open to solid tumors of varying tumor mutational burdens.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
31
Inclusion Criteria
  • Advanced or metastatic breast carcinoma, colorectal adenocarcinoma, uveal melanoma, cutaneous melanoma, non-small cell lung cancer, or head and neck squamous cell carcinoma that has failed or is refractory to standard of care therapy
  • Have at least one target lesion that can be used for response assessments and have at least 1 tumor amenable for tissue harvest for TIL manufacturing.
  • ECOG performance status of 0 or 1
  • Demonstrate adequate organ function
  • Additional inclusion criteria exist

Key

Exclusion Criteria
  • Known additional malignancy that is progressing or has required active treatment within the past 3 years
  • Diagnosis of immunodeficiency or receiving chronic systemic steroid therapy or any other form of immunosuppressive
  • Currently infected with HIV Type 1 and Type 2, hepatitis B virus (HBV), hepatitis C virus (HCV), treponema pallidum (e.g., syphilis), West Nile virus (WNV), Human T-lymphotropic virus types 1 or II (HTLV I/II), or cytomegalovirus (CMV)
  • Known active central nervous system (CNS) metastases and/or carcinomatous meningitis. Patients with previously treated brain metastases may participate provided they are radiologically stable
  • Serious cardiac condition, such as uncontrolled hypertension, concurrent congestive heart failure, prior history of Class III/IV cardiac disease (New York Heart Association [NYHA]), history of cardiac ischemia within the past 6 months, or prior history of cardiac arrhythmia requiring treatment. Patients who are > 60 years of age must undergo cardiology clearance exam and cardiac stress test.
  • Prior cell therapy or organ transplant
  • History of severe immediate hypersensitivity reaction to cyclophosphamide, fludarabine, IL-2, or pembrolizumab, or any of their constituents
  • LVEF ≤ 45%
  • FEV1 ≤ 60% of predicted value and DLCO (corrected) < 60% of predicted value
  • Chronic anti-coagulant therapy that cannot either be discontinued or temporarily changed
  • Additional exclusion criteria exist

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Breast CancerTBio-4101Patients with locally advanced or metastatic breast cancer that has failed or is intolerant to standard of care therapies. Includes, HER2+, HER 2-, TNBC.
Colorectal carcinomaTBio-4101Patients with advanced, metastatic colorectal adenocarcinoma who have failed or are intolerant to at least one line of therapy that included either irinotecan or oxaliplatin.
Uveal MelanomaTBio-4101Patients with advanced, metastatic uveal melanoma.
Non-Small Cell Lung CancerTBio-4101Patients with non-small cell lung cancer who have experienced disease progression following platinum containing chemotherapy and/or PD-1 or PD-L1 inhibitor.
Head and Neck Squamous Cell CarcinomaTBio-4101* Patients with head and neck squamous cell carcinoma who have received no prior therapy for metastatic disease * Patients with head and neck squamous cell carcinoma who are PD-1/PD-L1 inhibitor naïve at the time of TIL harvest and will be treated with TBio-4101 at the time of progression, inadequate response or intolerance to the PD-1/PD-L1 inhibitor-based standard of care regimen given on study.
Cutaneous MelanomaTBio-4101Patients with cutaneous melanoma who have experienced disease progression following a PD-1 or PD-L1 inhibitor.
Breast CancerPembrolizumabPatients with locally advanced or metastatic breast cancer that has failed or is intolerant to standard of care therapies. Includes, HER2+, HER 2-, TNBC.
Colorectal carcinomaPembrolizumabPatients with advanced, metastatic colorectal adenocarcinoma who have failed or are intolerant to at least one line of therapy that included either irinotecan or oxaliplatin.
Uveal MelanomaPembrolizumabPatients with advanced, metastatic uveal melanoma.
Cutaneous MelanomaPembrolizumabPatients with cutaneous melanoma who have experienced disease progression following a PD-1 or PD-L1 inhibitor.
Non-Small Cell Lung CancerPembrolizumabPatients with non-small cell lung cancer who have experienced disease progression following platinum containing chemotherapy and/or PD-1 or PD-L1 inhibitor.
Head and Neck Squamous Cell CarcinomaPembrolizumab* Patients with head and neck squamous cell carcinoma who have received no prior therapy for metastatic disease * Patients with head and neck squamous cell carcinoma who are PD-1/PD-L1 inhibitor naïve at the time of TIL harvest and will be treated with TBio-4101 at the time of progression, inadequate response or intolerance to the PD-1/PD-L1 inhibitor-based standard of care regimen given on study.
Primary Outcome Measures
NameTimeMethod
Safety and tolerability25 months

The incidence of treatment-emergent adverse events will be tabulated using NCI CTCAE v5.0

Secondary Outcome Measures
NameTimeMethod
Proportion of patients with a response (ORR)25 months

Percentage of all patients and within each cancer indication with a CR or PR as assessed by the independent central radiologist using RECIST 1.1 and iRECIST

Estimated Disease Control Rate (DCR)25 months

Portion of patient whose best response is a CR, PR, or stable disease (SD) as assessed by the independent central radiologist using RECIST v1.1 and iRECIST

Estimated Duration of Response (DoR)25 months

Duration of response, as measured in weeks, that patients with a CR or PR have no progressed (PD), as assessed by the independent central radiologist using RECIST v1.1 and iRECIST,

Trial Locations

Locations (13)

University of Miami

🇺🇸

Miami, Florida, United States

University of Chicago

🇺🇸

Chicago, Illinois, United States

Norton Cancer Institute

🇺🇸

Louisville, Kentucky, United States

MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

Princess Margaret Cancer Centre

🇨🇦

Toronto, Ontario, Canada

Moffitt Cancer Center

🇺🇸

Tampa, Florida, United States

Medical College of Wisconsin

🇺🇸

Milwaukee, Wisconsin, United States

Orlando Health

🇺🇸

Orlando, Florida, United States

Providence Cancer Institute

🇺🇸

Portland, Oregon, United States

Allegheny Research Institute

🇺🇸

Pittsburgh, Pennsylvania, United States

University of California Irvine

🇺🇸

Irvine, California, United States

Memorial Healthcare System

🇺🇸

Hollywood, Florida, United States

Montreal University Hospital Center

🇨🇦

Montréal, Quebec, Canada

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