House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

Not Applicable

Withdrawn

• Conditions: Allergic Asthma
• Interventions: Biological: House dust mite

• Registration Number: NCT03307278
• Lead Sponsor: China Medical University Hospital

Brief Summary

In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2017-09-26
• Study First Submitted QC: 2017-10-05
• Study First Posted: 2017-10-11
• Study Start: 2017-11-30
• Primary Completion: 2018-09-06
• Study Completion: 2018-09-06
• Status Verified: 2021-07
• Last Update Submitted: 2021-07-27
• Last Update Posted: 2021-08-02

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• HDM allergic and non allergic subjects

Exclusion Criteria

• Pregnant subject, cancer

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Steroid resistance in non HDM allergic subjects	House dust mite	-
Steroid resistance in HDM allergic patients	House dust mite	-

Primary Outcome Measures

Name	Time	Method
inflammasome activation by HDM	6 hours	The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
Steroid receptor phosphorylation by HDM	6 hours	The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.