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Vaccination with dendritic cells (DC) obtained from blood and loaded with fluorine particles and proteins derived from tumor cells of patients with stage III melanoma in whom a radical lymph node dissection is planned, and visualization of migration in vivo

Phase 1
Conditions
melanoma patients with regional lymph node metastases (stage III)
Therapeutic area: Diseases [C] - Skin and Connective Tissue Diseases [C17]
Registration Number
EUCTR2010-023757-11-NL
Lead Sponsor
Radboud University Nijmegen Medical Centre
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
ot Recruiting
Sex
All
Target Recruitment
28
Inclusion Criteria

-stage III melanoma with enlarged lymph nodes according to the 2001 AJCC criteria
- cytological or histological documented evidence of stage III melanoma
- HLA-A2.1 positive
- WHO performance status 0-1 (Karnofsky 100-70)
- life expectancy =3 months
- age 18-75 years
- no clinical signs or symptoms of CNS metastases
- WBC >3.0×109/l, lymphocytes >0.8×109/l, platelets >100×109/l, serum creatinine <150 µmol/l, serum bilirubin <25 µmol/l
- normal serum LDH (=250 U/l)
- expected adequacy of follow-up
- no pregnant or lactating women
- written informed consent
- radical regional lymphnode dissection is scheduled, but not yet performed
Are the trial subjects under 18? no
Number of subjects for this age range:
F.1.2 Adults (18-64 years) yes
F.1.2.1 Number of subjects for this age range 20
F.1.3 Elderly (>=65 years) yes
F.1.3.1 Number of subjects for this age range 8

Exclusion Criteria

- any prior chemotherapy, immunotherapy or radiotherapy is allowed if completed
more than 4 weeks prior to planned vaccination
- history of any second malignancy in the previous 5 years, with the exception of adequately treated basal cell carcinoma or carcinoma in situ of the cervix
- serious active infections, known HbsAg or HIV positive, or autoimmune diseases or organ allografts
- concomitant use of immunosuppressive drugs
- known allergy to shell fish (since it contains KLH)
- rapidly progressive symptomatic disease
- acute kidney injury or chronic severe kidney disease
- any serious clinical condition that may interfere with the safe administration of DC
- expected adequacy of follow-up
- no pregnant or lactating women
- written informed consent
- radical regional lymphnode dissection is scheduled

Study & Design

Study Type
Interventional clinical trial of medicinal product
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Secondary Outcome Measures
NameTimeMethod
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