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Immune Modulation Study in Patients With Metastatic Melanoma Treated With Anti-PD1 Monoclonal Antibodies

Phase 4
Conditions
Metastatic Melanoma
Interventions
Biological: blood sampling
Registration Number
NCT02626065
Lead Sponsor
Hospices Civils de Lyon
Brief Summary

This is an open mono-centric prospective non-randomized study in patients with metastatic melanoma treated with Anti-PD1 monoclonal antibodies (Nivolumab). The aim of the study is to identify the immune cells modulations differences between patients who present a complete, partial or stable response and patients who have non-response to the therapy in order to establish an improving response rate strategy.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
32
Inclusion Criteria
  • Men and women aged ≥ 18 years
  • Patient with metastatic or unresectable melanoma
  • Anti-PD1 monoclonal antibodies treatment indication
  • Patient affiliated to a social security regime
  • Signed Written Informed Consent.
  • agree with the storage of his biological samples
  • Women of childbearing potential must as mentioned in the summary of product characteristics (SPC) using two effective methods of contraception during treatment, and men whose partner is of childbearing potential must use effective contraception during treatment. For all patients treated men and women, contraception should be continued during the four months following the discontinuation of nivolumab.
Exclusion Criteria
  • development of haematological tumor during treatment
  • Patients requiring concomitant chronic treatment with systemic corticosteroids or other immunosuppressive agents
  • Patients with autoimmune disease.
  • Patient with Occular melanoma

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Nivolumab, patients with BRAF mutationblood samplingNivolumab (dose equal to 3mg/kg), 10 mg/ml solution for infusion. injection of Nivolumab every two weeks from day 0 and until relapse, toxicity motivating withdrawal or temporary suspension of treatment or up to 54 weeks. Blood sampling at different time
Nivolumab, patients with BRAF mutationNivolumabNivolumab (dose equal to 3mg/kg), 10 mg/ml solution for infusion. injection of Nivolumab every two weeks from day 0 and until relapse, toxicity motivating withdrawal or temporary suspension of treatment or up to 54 weeks. Blood sampling at different time
Nivolumab, patients with BRAF wild typeblood samplingNivolumab (dose equal to 3mg/kg), 10 mg/ml solution for infusion. injection of Nivolumab every two weeks from day 0 and until relapse, toxicity motivating withdrawal or temporary suspension of treatment or up to 54 weeks. Blood sampling at different time
Nivolumab, patients with BRAF wild typeNivolumabNivolumab (dose equal to 3mg/kg), 10 mg/ml solution for infusion. injection of Nivolumab every two weeks from day 0 and until relapse, toxicity motivating withdrawal or temporary suspension of treatment or up to 54 weeks. Blood sampling at different time
Primary Outcome Measures
NameTimeMethod
change the absolute number of dendritic cells before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

absolute number / mm 3 of dendritic cells

change the percentage of cells producing cytokines in dendritic cells before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

% of cells producing cytokines in dendritic cells

change the percentage of cells producing cytokines in monocytes before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

% of cells producing cytokines in monocytes

change the percentage of cells producing cytokines in subpopulations of T lymphocytes before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

% of cells producing cytokines in subpopulations of T lymphocytes

change the absolute number of subpopulations of T lymphocytes before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

absolute number / mm 3 of different subpopulations of T lymphocyte

change the absolute number of monocytes before treatment and on treatmentbefore treatment (week 0), at week 2, at week 12, at week 54 or at relapse (before 54 weeks)

absolute number / mm 3 of monocytes

Secondary Outcome Measures
NameTimeMethod
correlation between the occurrence of autoimmune side effects and the biological parameters before and on treatmentoccurence of autoimmune side effects from day 0 to week 54

absolute number / mm 3 of different population of cells and % of cells producing cytokines in the different population of cells will be correlated with autoimmune side effects, based on CTCAE classification

correlation between biological parameters and progression-free survivalprogression between the date of first injection of immunotherapy and week 54 based on RECIST and ir-RECIST criterion

absolute number / mm 3 of different population of cells and % of cells producing cytokines in the different population of cells will be correlated with the progression free survival

impact of treatments received prior to inclusion in the study on the biological parameters before and on treatmentantitumor treatment received from diagnosis of melanoma to inclusion

absolute number / mm 3 of different population of cells and % of cells producing cytokines in the different population of cells will be correlated with treatments received prior to inclusion

correlation between biological parameters on overall survivaldeath between the date of first injection of immunotherapy and week 54

absolute number / mm 3 of different population of cells and % of cells producing cytokines in the different population of cells will be correlated with the overall survival

Identify predictive factors of overall response rate at week 12 based on RECIST and ir-RECIST criteriaresponse evaluation at week 12

predictive factors like histological and cytological initial tumor type, initial immunological status will be evaluated at inclusion and correlated with the response

Trial Locations

Locations (1)

Centre Hospitalier Lyon Sud

🇫🇷

Pierre Benite, France

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