Human Intestinal Amino Acid Absorption and the Role of a Local (Renin)-Angiotensin System (RAS)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 50
- 试验地点
- 2
- 主要终点
- messenger ribonucleic acid (mRNA) quantification in intestinal tissue biopsy
研究概览
简要总结
This study is to find out what role a local intestinal RAS (renin angiotensin system) plays in the context of amino acid absorption in the human intestinal tract and how this RAS and thus the amino acid absorption is influenced by the RAS-active drugs (angiotensin II AT1 receptor blockers (sartans) or ACE inhibitors).
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Body Mass Index (BMI): 18-35 kg/m2
- •cohort 1: therapy with an angiotensin II AT1 antagonist or ACE Inhibitor
- •cohort 2: no therapy with an angiotensin II AT1 antagonist or ACE Inhibitor
- •Carrying out a diagnostic gastroscopy, colonoscopy or a combined gastroscopy / colonoscopy
- •Existence of written consent after detailed information about the study
排除标准
- •Severe pathological changes in the gastrointestinal tract (e.g. sprue, stomach ulcers, malignancies); (reflux esophagitis and gastritis are not Exclusion criteria)
- •History of gastrointestinal tract surgery (except for appendectomy and inguinal hernia surgery)
- •History of malignancy
- •Severe acute and chronic organ diseases requiring treatment (e.g. kidney replacement therapy)
- •Patients with an increased risk of bleeding (e.g. oral anticoagulation, coagulation disorders)
- •Drug or alcohol abuse
- •Mental impairment limiting the ability to meet all study requirements
结局指标
主要结局
messenger ribonucleic acid (mRNA) quantification in intestinal tissue biopsy
时间窗: single time-point at baseline
mRNA quantification in intestinal tissue biopsy: mRNA content coding for regulatory RAS protein is measured relative to the mRNA content, which codes for the structural protein villin.
amino acid concentration in blood sample
时间窗: single time-point at baseline
amino acid concentration in blood sample by High Performance Liquid Chromatography (HPLC)
amino acid concentration in urine sample
时间窗: single time-point at baseline
amino acid concentration in urine sample by HPLC (High Performance Liquid Chromatography)
次要结局
未报告次要终点