The Influence of Age and Physical Fitness Level on the Level of Energy Metabolism and Antioxidant Capacity in Adult Males
Overview
- Phase
- N/A
- Intervention
- Not specified
- Conditions
- Healthy
- Sponsor
- Poznan University of Physical Education
- Enrollment
- 420
- Locations
- 1
- Primary Endpoint
- Cardio-respiratory measures
- Status
- Completed
- Last Updated
- 4 years ago
Overview
Brief Summary
The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.
Detailed Description
Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.
Investigators
Eligibility Criteria
Inclusion Criteria
- •non-smoking
- •physically fit, including top-level athletes
Exclusion Criteria
- •no reported history of a cardiopulmonary or renal diseases, and other chronic diseases
- •no major orthopedic injury or illness resulting in an inability to run
- •no medications that could affect cardiopulmonary functions
- •normal resting electrocardiogram
- •body mass index (BMI) below 30.0 kg·m-2
- •normal erythrocyte count and Hb content
- •no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency
Outcomes
Primary Outcomes
Cardio-respiratory measures
Time Frame: during the intervention
Cardiopulmonary exercise test (CPET) was conducted while an incremental running test on the treadmill using a portable breath-by-breath ergospirometer and heart rate monitor.
Secondary Outcomes
- Glutathione peroxidase (GPx) activity(baseline)
- Blood Lactate level(baseline and immediately after the intervention)
- Total antioxidant status (TAS)(baseline)
- Plasma purine nucleotides(baseline and immediately after the intervention)
- Erythrocyte Purine Metabolites(baseline and immediately after the intervention)
- Malondialdehyde (MDA)(baseline)
- Aminoacid profile(baseline)
- Nicotinamide Adenine Dinucleotide (NAD) and its metabolites(baseline and immediately after the intervention)
- Reduced glutathione (GSH)(baseline)
- Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity(baseline and immediately after the intervention)
- Thiobarbituric-acid-reactive substances (TBARS)(baseline)
- Polyphenols(baseline)