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Effect of MEDiterranean Diet on the microBIOME of Individuals With Type 2 Diabetes

Not Applicable
Conditions
Type 2 Diabetes
Interventions
Other: Individualized structured dietary plan based on MD
Registration Number
NCT04403217
Lead Sponsor
Universidade Nova de Lisboa
Brief Summary

Dysbiosis of gut microbiota is associated with the pathophysiology of type 2 diabetes. Mediterranean diet has been indicated as a dietary pattern that promote the integrity of gut barrier and that has positive impact in the metabolic control of subjects with type 2 diabetes. This is a 12-week, single-arm clinical study that aims to evaluate if the effect of Mediterranean diet in metabolic control of these subjects is mediated by gut microbiota. Subjects will receive personalized nutrition counseling where Mediterranean diet will be promoted.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Men and women diagnosed with type 2 diabetes mellitus, according to the American Diabetes Association criteria;
  • Ages 40-80 years;
  • Non-smoker;
  • Willing and able to provide written informed consent.
Exclusion Criteria
  • Diabetes diagnosed before 40 years old;
  • Changes in oral glycaemic-control medications in the last 3 months;
  • Subjects with HbA1c levels under 6.4% or above 10%;
  • Subjects under insulinotherapy;
  • Subjects under corticotherapy;
  • Subjects with triglycerides levels above 4.52 nmol/L (400 mg/dL);
  • Intake of antibiotics in the last 12 weeks;
  • Subjects with a diagnosis of any digestive disease including functional bowel disorders such as IBS.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Individualized structured dietary plan based on MDIndividualized structured dietary plan based on MDParticipants will follow an individualized structured dietary plan based on Mediterranean diet for 12 weeks
Primary Outcome Measures
NameTimeMethod
Changes in HOMA-IR from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in intestinal microbiota from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2) and 12-week post Mediterranean diet intervention (visit 3)

Bacterial DNA will be extracted from fecal samples. 16SRNA gene will be sequenced by next-generation sequencing (NGS). All the identified bacterial phyla, genus and species will be expressed in percentage

Changes in fasting insulin (μU/mL) levels from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in HbA1c levels (percent) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in HOMA-B (percent) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in HOMA-S (percent) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in fasting capillary blood glucose (mg/dL) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Secondary Outcome Measures
NameTimeMethod
Changes in free fat mass (kg) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in total body fat mass (kg) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in diet quality from baseline to visit 31-week post run-in period (baseline), 12-week post Mediterranean diet intervention (visit 3)

Mediterranean diet adherence will be evaluated using the Mediterranean Diet Adherence Screener (MEDAS) from the PREvención com Dieta MEDiterránea (PREDIMED) study. Responses that are favorable to the adoption of the Mediterranean diet are scored as 1 point, while responses that are unfavorable are scored as 0.

The final score ranged between 0 and 14 and a MEDAS score greater than 9 indicates high adherence to the Mediterranean diet.

Changes in food intake from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)

3 non-consecutive 24-h dietary food recall using a photographic manual for food quantification

Changes in fasting serum total cholesterol (mg/dL) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in fasting serum low-density lipoprotein (LDL) (mg/dL) cholesterol from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in fasting serum triglycerides (mg/dL) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in fasting serum high-density lipoprotein (HDL) (mg/dL) cholesterol from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)
Changes in waist perimeter (cm) from baseline to visit 2 and 31-week post run-in period (baseline), 4-week post Mediterranean diet intervention (visit 2), 12-week post Mediterranean diet intervention (visit 3)

Trial Locations

Locations (1)

NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa

🇵🇹

Lisbon, Portugal

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