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Effect of Metformin on Healthy Live Birth in Women With Prediabetes

Not Applicable
Recruiting
Conditions
Metformin
Prediabetes
In-Vitro Fertilization
Interventions
Drug: Placebo pretreatment before ovarian stimulation
Drug: Placebo pretreatment before endometrial preparation for frozen embryo transfer
Registration Number
NCT06064669
Lead Sponsor
Shandong University
Brief Summary

To evaluate the efficacy and safety of metformin pretreatment on reproductive outcomes in infertile women with prediabetes.

Detailed Description

To evaluate the efficacy and safety of metformin pretreatment on reproductive outcomes in infertile women with prediabetes and to determine whether either starting metformin pretreatment before ovarian stimulation (aiming at improving the quality of oocyte/embryo) or starting before frozen embryo transfer (FET) (aiming at improving the receptivity of endometrium) could increase the chance of a healthy live birth compared with placebo.

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
988
Inclusion Criteria
  1. Women who are diagnosed with prediabetes by ADA criteria, including either IFG, IGT, or HbA1C 5.7-6.4%.
  2. Women aged 20-40 years.
  3. Women who plan to undergo a new cycle of IVF, ICSI, or PGT-A.
Exclusion Criteria
  1. Women who are diagnosed with diabetes according to the ADA criteria11,12, which is meeting one of the following criteria: fasting plasma glucose ≥7.0mmol/L, 2-h plasma glucose during 75-g OGTT ≥11.1mmol/L, HbA1c≥6.5%, or a random plasma glucose≥11.1mmol/L.
  2. Women who are taking medicine that interfere with glucose metabolism, such as metformin, oral anti-diabetic agents (sulfonylureas, glinides, thiazolidinediones, α-glycosidase inhibitors, GLP-1 receptor agonist, etc.), weight loss drugs (i.e.orlistat, etc.), glucocorticoids, and growth hormones within 2 months before enrollment.
  3. Women with un-corrected hyperthyroidism or hypothyroidism.
  4. Women with congenital or acquired abnormal uterine cavity including septate uterus, unicornous uterus, uterus duplex, and intrauterine adhesions.
  5. Women with a diagnosis of adenomyosis.
  6. Women with untreated hydrosalpinx.
  7. Women who plan to undergo PGT-SR or PGT-M.
  8. Women with major medical comorbidities, such as known liver disease, known renal disease, or known significant anemia.

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
Metformin-Metformin GroupMetformin pretreatment before endometrial preparation for frozen embryo transferMetformin pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by metformin pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Placebo-Metformin GroupMetformin pretreatment before endometrial preparation for frozen embryo transferPlacebo pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by metformin pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Metformin-Metformin GroupMetformin pretreatment before ovarian stimulationMetformin pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by metformin pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Metformin-Placebo GroupMetformin pretreatment before ovarian stimulationMetformin pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by placebo pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Placebo-Placebo GroupPlacebo pretreatment before endometrial preparation for frozen embryo transferPlacebo pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by placebo pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Placebo-Metformin GroupPlacebo pretreatment before ovarian stimulationPlacebo pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by metformin pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Metformin-Placebo GroupPlacebo pretreatment before endometrial preparation for frozen embryo transferMetformin pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by placebo pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Placebo-Placebo GroupPlacebo pretreatment before ovarian stimulationPlacebo pretreatment before ovarian stimulation till oocyte retrieval and cryopreservation of all embryos, and followed by placebo pretreatment before endometrial preparation for frozen embryo transfer and till the establishment of clinical pregnancy (7-8 weeks gestation) after the first frozen embryo transfer.
Primary Outcome Measures
NameTimeMethod
healthy live birthFrom the date of randomization until delivery after the first embryo transfer, up to 16 months

defined as a singleton live birth at ≥37 weeks, with infant birth weight between 2500 and 4000g and without a major congenital anomaly.

Secondary Outcome Measures
NameTimeMethod
Gestational weight gainat delivery

maternal weight gain during pregnancy

Change in 2-h levels of glucose after 75-g OGTTFrom the date of randomization until the establishment of clinical pregnancy after the first embryo transfer, up to 26 weeks

change in 2-h levels of glucose after 75-g OGTT from baseline to the establishment of clinical pregnancy.

Days of ovarian stimulationFrom the date of randomization until the day of oocyte retrieved, up to 12 weeks

the duration of ovarian stimulation by exogenous gonadotropin

Change in BMIFrom the date of randomization until the establishment of clinical pregnancy after the first embryo transfer, up to 26 weeks

change in body mass index (BMI) from baseline to the establishment of clinical pregnancy.

Pregnancy lossFrom the date of confirmation of pregnancy until the date of pregnancy loss, up to 9 months

defined as pregnancies that eventuate in a spontaneous abortion or therapeutic abortion that occurred throughout pregnancy.

Live birthFrom the date of randomization until delivery after the first embryo transfer, up to 16 months

defined as the delivery of any neonate with signs of life at ≥ 28 weeks of gestation

OHSSFrom the day of oocyte retrieved until the initiation of endometrial preparation for the first frozen embryo transfer, up to 8 weeks

ovarian hyperstimulation syndrome was defined according to the Golan criteria

LBWat delivery

the infant born weighing less than 2500g

Change in fasting glucose levelFrom the date of randomization until the establishment of clinical pregnancy after the first embryo transfer, up to 26 weeks

change in fasting glucose level from baseline to the establishment of clinical pregnancy.

Change in fasting insulin levelFrom the date of randomization until the establishment of clinical pregnancy after the first embryo transfer, up to 26 weeks

change in fasting insulin level from baseline to the establishment of clinical pregnancy.

Peak estradiol levelFrom the date of randomization until the day of oocyte retrieved, up to 12 weeks

the estradiol level on the day of hCG trigger

Number of euploid embryosFrom the date of randomization until the initiation of endometrial preparation for frozen embryo transfer, up to 20 weeks

the number of euploid embryos

Clinical pregnancy30-35 days after the first frozen embryo transfer

defined as the ultrasound confirmation of at least one intrauterine gestational sac

Change in 2-h levels of insulin after 75-g OGTTFrom the date of randomization until the establishment of clinical pregnancy after the first embryo transfer, up to 26 weeks

change in 2-h levels of insulin after 75-g OGTT from baseline to the establishment of clinical pregnancy.

Total dose of gonadotropinsFrom the date of randomization until the day of oocyte retrieved, up to 12 weeks

the total dose of exogenous gonadotropin used during ovarian stimulation

Number of oocyte retrievedFrom the date of randomization until the day of oocyte retrieved, up to 12 weeks

the number of oocyte retrieved

Number of good-score embryosFrom the date of randomization until the third day after oocyte retrieval, up to 12 weeks

the number of good-score embryos

LGAat delivery

defined as the birthweight above the 90th percentile for gestational age based on a sex-specific reference

Macrosomiaat delivery

the infant born weighing larger than 4000g

Singleton or twin pregnancy30-35 days after the first frozen embryo transfer

the number of intrauterine gestational sacs

Incidences of obstetric and neonatal complicationsFrom the establishment of clinically recognized pregnancy until six weeks after delivery after the first embryo transfer, up to 11 months

including gestational diabetes mellitus, pre-eclampsia, premature rupture of membrane, placenta previa, placental abruption, congenital anomalies, postpartum hemorrhage, stillbirth, neonatal respiratory distress syndrome, neonatal jaundice, neonatal infection, neonatal death.

SGAat delivery

defined as the birthweight below the 10th percentile for gestational age based on a sex-specific reference

Birth weightat delivery

birth weight of the newborn at delivery

Adverse eventsFrom the date of randomization until six weeks after delivery after the first embryo transfer, up to 18 months

adverse event (AE) and serious adverse event (SAE)

Cumulative live birthFrom the date of randomization until delivery, up to 28 months

define as women achieving live birth after all the cycles of embryo transfer per oocyte retrieval that performed within one year after randomization.

Trial Locations

Locations (1)

Shandong University

🇨🇳

Jinan, Shandong, China

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