To study the efficacy of five mouthwashes in reducing viral load and bacterial colonies in oral cavity

Not yet recruiting

• Conditions: Healthy Human Volunteers without any systemicillness

• Registration Number: CTRI/2022/08/044814
• Lead Sponsor: Dr Ekta Sharma

Brief Summary

Aim : To evaluate the efficacy of different mouthwashes for reducing viral load and bacterial colonies in oral cavity .

Methodology:  75 patients coming to Department of Orthodontics and Dentofacial

Orthopaedics, Inderprastha Dental College and Hospital for orthodontic treatment

will be considered for the study.

All the volunteers were divided into five groups: 15 subjects per group.

Subjects’ saliva was collected early in the morning after normal oral hygienic

procedures.

Spitting method was employed in which the subjects were seated with head

inclined forward so that the saliva will collect in the floor of the mouth from

where it is spat into a 20 ml sterile specimen bottle for 5 minutes.

The saliva samples collected in the before the rinse for each group served as the

control and the samples collected after 5minutes of use mouth rinse

chlorhexidine, ,hydrogen peroxide, cetylpyridinium chloride and povidine-iodine

as the experimental..

All five Mouthwashes were used in definite concentration

1.chlorhexidine brand name chlorhex-0.2%

2. hydrogen peroxide brand name hydroxyl-3%

3.cetylpyridinium chloride brand name mcclean-0.07%

4. Povidine-iodine brand name betadine-7%

5. Colgate Plax

Subects in each group will be asked to rinse their mouth using respective mouthwashes and their samples will be collected after two hours of rinsing using saliva collection kit during first visit and one month after bonding. Sample will be stored using dry ice at - 80 degree. Then sample will be tested for the load of rhinovirus using ELISA and bacterial load will be detected by measuring CFU on blood agar medium.

Detailed Description

Not available

Study Timeline

• Last Update Posted: 2022-08-22
• Study Start: 2022-08-28

Recruitment & Eligibility

• Status: Not Yet Recruiting
• Sex: All
• Target Recruitment: 75

Inclusion Criteria

Healthy individual understanding and accepting trial.

Exclusion Criteria

• Individuals on medication or having any systemic illness.
• Pregnant or lactating females.
• Patients already using mouthwash regularly more than once a week.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
the expected primary outcome of this study is that group 2(hydrogen peroxide)will reduce the viral load in oral cavity the more as compared to other groups in the samples collected pre and post bonding.	4weeks

Secondary Outcome Measures

Name	Time	Method
the expected secondary outcome is to check that group 2(hydrogen peroxide) will reduce bacterial colonisation more as compared to the other groups	4 weeks

Trial Locations

Locations (1)

Inderprastha Dental College & Hospital; 🇮🇳 Ghaziabad, UTTAR PRADESH, India

Inderprastha Dental College & Hospital

🇮🇳Ghaziabad, UTTAR PRADESH, India

Dr Vineet Golchha

Principal investigator

9990156222

orthovineet@gmail.com