The Importance of Blastocyst Diamater and Its Correlation to Reproductive Outcomes in PGT-A Cases
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 1,000
- 试验地点
- 1
- 主要终点
- Blastocoele re-expansion degree
研究概览
简要总结
Morphometric measurement of blastocyst diameter was taken on the day of trophoectoderm biopsy. PGT-A was done using NGS platform, followed by thawing and transfer of euploid blastocyts. Follow up of the Pregnancy and implantation rates is done and recorded to study the correlation between the diameter and reproductive outcomes of the blastocyst.
详细描述
Blastocyst morphological grading proposed by Gardner and Schoolcraft including the expansion degree, inner cell mass, and trophoectoderm assessment has proved its importance. Blastocyst expansion degree that can presented in diameter is linked to the blastocyst implantation and overall success.
In order to evaluate the importance of blastocyst diameter in PGT-A cases, measurements will be taken on the day of trophoectoderm biopsy TE (diameter in μm), followed by TE biopsy using flicking technique. Next, PGT-A will be done using NGS platform and results will be confirmed using PGT-ai. Thawing of euploid blastocyst will take place and expansion degree, survival rate will be recorded. Patient will be followed up for the clinical pregnancy and implantation rates.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Female aged 18-45 years old
- •Male aged 18-60 years old
- •ICSI cases having PGT-A for their blastocysts
- •Must have their blastocysts cultured in time lapse incubator
排除标准
- •Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection
- •Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation
- •Use of donor oocytes or sperm
- •Use of gestational carrier
结局指标
主要结局
Blastocoele re-expansion degree
时间窗: Two hours post thawing
Defined as the degree of blastocoele re-expansion and presented in %
Implantation rate
时间窗: 6-8 weeks of pregnancy
Defined as number of gestational sacs with fetal heart beat, shown by ultrasound in gestational week 6 over number of embryo transferred.
Pregnancy rate
时间窗: 15 days post embryo transfer
Defined as clinical pregnancy per transfer
Blastocyst survival rate
时间窗: Two hours post thawing
Defined as the proportion of blastocysts that showed expansion degree ≥50% and their cells remained intact after thawing and re-expansion
次要结局
未报告次要终点
研究者
Eman Hasanen
Embryologist
Ganin Fertility Center