Phase I Study of Autologous CAR T-Cells Targeting the CD138 Antigen for Relapsed or Refractory Multiple Myeloma
试验速览
- 阶段
- 1 期
- 状态
- 进行中（未招募）
- 入组人数
- 25
- 试验地点
- 2
- 主要终点
- Proportion of participants with dose limiting toxicities as a measure of maximum tolerated dose of CAR138 T cells
研究概览
简要总结
The body has different ways of fighting infection and disease. No single way seems perfect for fighting cancer. This research study combines two different ways of fighting disease: antibodies and T cells. Antibodies are proteins that protect the body from disease caused by bacteria or toxic substances. Antibodies work by binding those bacteria or substances, which stops them from growing and causing bad effects. T cells, also called T lymphocytes, are special infection-fighting blood cells that can kill other cells, including tumor cells or cells that are infected. Both antibodies and T cells have been used to treat subjects with cancers. They both have shown promise, but neither alone has been sufficient to cure most subjects. This study is designed to combine both T cells and antibodies to create a more effective treatment. The treatment that is being researched is called autologous T lymphocyte chimeric antigen receptor cells targeted against the CD138 antigen (CAR138 T cells).
In previous studies, it has been shown that a new gene can be put into T cells that will increase their ability to recognize and kill cancer cells. A gene is a unit of DNA. Genes make up the chemical structure carrying the subject's genetic information that may determine human characteristics (i.e., eye color, height and sex). The new gene that is put in the T cells in this study makes a piece of an antibody called anti-CD138. This antibody floats around in the blood and can detect and stick to cancer cells called multiple myeloma cells because they have a substance on the outside of the cells called CD138. Anti-CD138 antibodies have been used to treat people with multiple myeloma, but have not been strong enough to cure most subjects. For this study, the anti-CD138 antibody has been changed so that instead of floating free in the blood part of it is now joined to the T cells. Only the part of the antibody that sticks to the multiple myeloma cells is attached to the T cells instead of the entire antibody. When an antibody is joined to a T cell in this way it is called a chimeric receptor. These CD138 chimeric (combination) receptor-activated T cells seem to kill some of the tumor, but they do not last very long in the body and so their chances of fighting the cancer are unknown.
详细描述
Cell Procurement Up to 300 mL total of peripheral blood (up to 3 collections) will be obtained from subjects for cell procurement. In subjects with a low CD3 count in the peripheral blood (less than 200/μl by flow cytometry), a leukopheresis may be performed to isolate sufficient T-cells. The parameters for pheresis will be 2 blood volumes.
CAR138 T-cell Administration Autologous CAR138 T-cells will be administered 2-14 days following lymphodepletion. The lymphodepletion regimen will consist of Cyclophosphamide 300 mg/m^2 and Fludarabine 30 mg/m^2 IV each given daily over 3 consecutive days.
Duration of Therapy Autologous CAR138 T-cells will be administered 2-14 days following lymphodepletion with cyclophosphamide and fludarabine by a licensed provider (oncology registered nurse or physician) via intravenous injection over 5-10 minutes through either a peripheral or central line. The expected volume is 1-50cc.
Treatment with one infusion will be administered unless:
- The subject decides to withdraw from the study, OR
- General or specific changes in the subject's condition render the subject unacceptable for further treatment in the judgment of the investigator.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Eligibility criteria are divided into 3 sections: 1) eligibility criteria to be fulfilled prior to cell procurement, 2) Eligibility criteria to be met prior to lymphodepletion and 3) Eligibility criteria to be met prior to CAR138 T cell infusion.
- •Note: During the period of cell procurement and CAR138 T-cell production, subjects are allowed to receive additional standard of care chemotherapy or radiation therapy to stabilize their multiple myeloma if the treating physician feels it is in the subject's best interests. For subjects requiring bridging chemotherapy and/or radiation therapy while awaiting manufacture of their CAR138-T cells, details regarding treatment(s) administered including doses, frequency, number of cycles, etc. will be collected.
- •Eligibility criteria to be fulfilled prior to cell procurement
- •Subjects must fulfill all of the following criteria to participate in this study.
- •Written informed consent and HIPAA authorization for release of personal health information. Subjects must sign a consent to undergo cell procurement.
- •Age ≥ 18 years at the time of consent.
- •Karnofsky score of ≥ 60%.
- •Diagnosis of relapsed or refractory multiple myeloma (as defined by the Revised Uniform Response Criteria outlined by the IMWG
- •Measurable disease as defined by one or more of the following: 1) serum M-protein
- •≥1.0 g/dL (≥0.5 g/dL for IgA myeloma); 2) urine M-protein ≥200 mg/24 hours; 3) involved serum free light chain level ≥10 mg/dL AND an abnormal serum free light chain ratio. Subjects with non-secretory disease and a baseline marrow burden of myeloma of at least 30% will also be eligible to participate.
- •Received at least 3 lines of prior chemotherapy. The prior regimens must have included an immunomodulatory agent (lenalidomide or pomalidomide) and a proteasome inhibitor (bortezomib, carfilzomib or ixazomib).
- •Two lines of therapy will be allowed if the subject has disease that is refractory to both an immunomodulatory agent (lenalidomide or pomalidomide) and a proteasome inhibitor.
- •Received high dose melphalan followed by autologous stem-cell transplant (ASCT) or is not eligible for or has declined the procedure.
- •Allogeneic stem cell transplantation is allowed provided the subject is ≥ 1 year from immunosuppressive therapy to treat/prevent graft-versus-host disease, has no evidence of active graft-versus-host disease, and has no evidence of active infection.
- •Women of childbearing potential (WOCBP) should be willing to use 2 methods of birth control or be surgically sterile, or abstain from heterosexual activity for the course of the study (starting prior to procurement), and for 6 months after the study is concluded. WOCBP are those who have not been surgically sterilized or have not been free from menses for > 1 year. The two birth control methods can be composed of: two barrier methods or a barrier method plus a hormonal method to prevent pregnancy. The male partner of WOCBP subjects enrolled into the trial should use a condom and female participants must take the responsibility to inform their partners of the need to use a condom.
- •Not pregnant or breastfeeding (NOTE: breast milk cannot be stored for future use while the mother is being treated on study).
- •No tumor in a location where enlargement could cause airway obstruction.
- •No diagnosis of any of the following conditions: amyloidosis, POEMS syndrome or multiple myeloma with CNS involvement.
- •Subjects with plasma cell leukemia are allowed to participate.
- •No active inflammatory or infectious gastrointestinal disorder (e.g. infectious colitis, diverticulitis or inflammatory bowel disease).
- •No psychiatric illness which would prevent the subject from giving informed consent, or neurological illness that the clinician believes would complicate monitoring for CNS neurotoxicity following CAR-T infusion.
- •Subjects are willing and able to comply with study procedures based on the judgment of the investigator or protocol designee.
- •No medical condition, which, in the opinion of the treating physician, would make this protocol unreasonably hazardous for the subject.
- •No other prior or concomitant malignancies with the exception of:
- •Non-melanoma skin cancer
- •In-situ malignancy
- •Low-risk prostate cancer after curative therapy
- •Other cancer for which the subject has been disease free for ≥ 3 years.
- •Adequate cardiac function, defined as:
- •No ECG evidence of acute ischemia
- •No ECG evidence of active, clinically significant conduction system abnormalities
- •Prior to study entry, any ECG abnormality at screening not felt to put the subject at risk has to be documented by the investigator as not medically significant
- •No uncontrolled angina or severe ventricular arrhythmias
- •No clinically significant pericardial disease
- •No history of myocardial infarction within the last 6 months prior to registration
- •No Class 3 or higher New York Heart Association Congestive Heart Failure
- •No active infection (fungal, bacterial or viral) including HIV, HTLV, HBV, HCV (tests can be pending at the time of cell procurement; only those samples confirming lack of active infection will be used to generate transduced cells). Note: To meet eligibility, subjects are required to be negative for HIV antibody or HIV viral load, negative for HTLV1 and 2 antibody or PCR negative for HTLV1 and 2, negative for Hepatitis B surface antigen, and negative for HCV antibody or HCV viral load.
- •Demonstrate adequate organ function prior to cell procurement as defined below:
- •Creatinine Clearance using the Cockcroft-Gault formula: ≥ 50 mL/min within 60 days of cells procurement, must be ≥ 30 mL/min within 24 hours of procurement.
- •Bilirubin: ≤ 1.5 × upper limit of normal (ULN) unless attributed to Gilbert's syndrome
- •Aspartate aminotransferase (AST): ≤ 2.5 × ULN
- •Alanine aminotransferase (ALT): ≤ 2.5 × ULN
- •Oxygen saturation: ≥ 92% on room air
- •Ejection fraction: ≥ 45%
- •Platelets: ≥ 50,000 /mm^3; mm3 within 60 days of cell procurement, must be ≥ 20,000/mm3 within 24 hours of procurement.
- •ANC: ≥ 1000 /mm^3; within 60 days of cell procurement, must be ≥ 500/mm3 (0.5 × 109/L) within 24 hours of procurement.
- •Negative serum pregnancy test within 72 hours prior to cell procurement for female participants of childbearing potential. NOTE: Females are considered of childbearing potential unless they are surgically sterile (have undergone a hysterectomy, bilateral tubal ligation, or bilateral oophorectomy) or they are naturally postmenopausal for at least 12 consecutive months.
- •Subjects who have received prior CAR-T must be ≥9 months out from prior CAR-T and have no available or more suitable treatment options in the opinion of the treating investigator than this protocol.
- •Eligibility Criteria to be fulfilled prior to lymphodepletion
- •__________________________________________________ Written informed consent to enroll in the CAR T-cell therapy trial must be obtained prior to lymphodepletion.
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排除标准
- 未提供
研究组 & 干预措施
CAR138 T cells
The first 3 subjects enrolled in the study will receive 5x10^6 CAR138 T-cells/m^2 via infusion. The number of cells for the infusion will be increased to 1x10^7 CAR138 T-cells/m^2 and then, 2.5x10^7 CAR138 T-cells/m^2, 5x10^7 CAR138 T-cells/m^2, 1x10^8 CAR138 T-cells/m^2 and 2x10^8 CAR138 T-cells/m^2 in subsequent cohorts of 3 subjects provided no dose limiting toxicities (DLTs) are observed within 4 weeks of the cell infusion. Cohort enrollment will be staggered, requiring each subject to complete at least 2 weeks of safety monitoring following CAR138 T-cell infusion at the designated dose level for the cohort before another subject is allowed to enroll in the cohort.
干预措施: CAR138 T Cells (Drug)
结局指标
主要结局
Proportion of participants with dose limiting toxicities as a measure of maximum tolerated dose of CAR138 T cells
时间窗: 4 weeks from infusion of the CAR138 T cells
The maximum tolerated dose will be determined through assessment of dose limiting toxicity (DLT) experienced during the safety evaluation period. Severity and categorization of adverse events will be determined in accordance with the National Cancer Institute's Common Terminology for Adverse Events (CTCAE, version 5.0). A DLT is defined as any of the following that may, after consultation with the FDA, be considered possibly, probably, or definitely related to the study cellular products: Grade 3 and 4 cytokine release syndrome (CRS) that persists beyond 72 hours, or any Grade 5 CRS event; Grade 4 neutropenia or thrombocytopenia lasting more than 28 days from the time of CAR138 T-cell infusion. Any other ≥ Grade 3 non- hematologic toxicity (exceptions include: alopecia; grade 3 electrolyte abnormalities, hyperglycemia, diarrhea, or nausea and vomiting that can be managed with supportive care and do not persist as Grade 3 toxicities for \> 72 hours)).
次要结局
- Overall response rate (ORR) as defined as the percentage of subjects achieving a confirmed partial response or better based on the International Myeloma Working Group (IMWG) response criteria.(15 years)
- Survival of CAR138 T cells in vivo as assessed by PCR and flow cytometry(15 years)
- Overall survival after infusion of CAR138 T cells(15 years)
- Progression free survival after infusion of CAR138 T cells(15 years)