Interleukin-6 and Cortisol Responses Following Short- and Long-Term Cycling Tests in Team Athletes With Different Cardiorespiratory Fitness Levels
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 27
- 试验地点
- 1
- 主要终点
- Salivary Cortisol Response
研究概览
简要总结
This study aimed to investigate salivary interleukin-6 (IL-6) and cortisol responses following short-term and long-term cycling exercise in team athletes with different cardiorespiratory fitness levels. Participants completed a Wingate anaerobic cycling test and a YMCA submaximal cycling test in a randomized crossover design separated by a seven-day washout period. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise to determine IL-6 and cortisol concentrations. The study also examined whether cardiorespiratory fitness level influenced the magnitude of these physiological responses.
详细描述
Exercise-induced physiological stress activates numerous neuroendocrine and inflammatory pathways, including the hypothalamic-pituitary-adrenal axis and cytokine-mediated responses. Cortisol and interleukin-6 (IL-6) are among the most commonly investigated biomarkers of exercise stress and adaptation. However, the influence of cardiorespiratory fitness on acute hormonal and inflammatory responses to exercise remains incompletely understood.
This study employed a randomized crossover design involving team athletes aged 18-35 years. Participants completed two cycling exercise protocols: a short-term anaerobic test (Wingate) and a long-term aerobic test (YMCA submaximal cycling test). The two testing sessions were separated by a seven-day washout period and were performed at the same time of day to minimize circadian variation.
Saliva samples were collected before exercise, immediately after exercise, and 30 minutes post-exercise. Salivary IL-6 and cortisol concentrations were analyzed to determine acute physiological responses to both exercise modalities. Participants were categorized according to cardiorespiratory fitness level based on estimated VO₂max values, and biomarker responses were compared between fitness groups.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Team athletes aged 18 to 35 years Participation in organized team sports (e.g., volleyball, basketball, soccer, or handball) Minimum of 3 training sessions per week, with each session lasting at least 1 hour Sports participation history of at least 2 years Apparently healthy and free from known cardiovascular, metabolic, endocrine, or respiratory diseases Willingness to provide written informed consent
排除标准
- •Current musculoskeletal injury or illness affecting exercise performance Use of medications known to influence hormonal or inflammatory responses Smoking or use of tobacco products Acute infection within the previous two weeks Failure to complete both exercise testing sessions Failure to comply with pre-test instructions
研究组 & 干预措施
Long-Term Cycling Test
Participants performed a YMCA submaximal cycling test to assess cardiorespiratory fitness. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise for the assessment of cortisol and interleukin-6 responses.
干预措施: YMCA Submaximal Cycling Test (Behavioral)
Short-Term Cycling Test
Participants performed a 30-second Wingate anaerobic cycling test. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise for the assessment of cortisol and interleukin-6 responses.
干预措施: Wingate Anaerobic Cycling Test (Behavioral)
结局指标
主要结局
Salivary Cortisol Response
时间窗: Pre-exercise, immediately post-exercise, and 30 minutes post-exercise
Change in salivary cortisol concentrations measured before exercise, immediately after exercise, and 30 minutes after exercise following short-term and long-term cycling tests.
次要结局
- Salivary Interleukin-6 (IL-6) Response(Pre-exercise, immediately post-exercise, and 30 minutes post-exercise)
研究者
Eda Karaduman
Research Assistant, PhD
Ondokuz Mayıs University