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NiPPeR Randomised Trial - Child Follow Up Study

Not Applicable
Conditions
Cognition
Atopic Dermatitis
Lifestyle
Microbial Colonization
Allergy
Neurobehavioral Manifestations
Metabolic Disturbance
Nutrition, Healthy
Breastfeeding
Growth Disorders
Interventions
Other: Intervention (Study Nutritional Drink)
Other: Control (Standard Nutritional Drink)
Registration Number
NCT05934422
Lead Sponsor
University of Southampton
Brief Summary

There is an increasing focus on the need to optimise nutrition, lifestyle and metabolism of parents before and during pregnancy and of the infant after birth, but as yet there is limited understanding of the specific influences and of the underlying mechanisms.

This study is a follow up of children from the NiPPeR trial of a nutritional drink enriched with micronutrients, myo-inositol and probiotics taken preconception and during pregnancy. In this setting we will examine the influence of parental nutrition, lifestyle and metabolism before and during pregnancy on child growth, development and well-being; ascertaining growth, adiposity, metabolism, neurobehavioural and health outcomes in the children, and characterising the underlying mechanisms.

The data collected will allow identification of the contributions of parental and offspring characteristics, nutritional, lifestyle and medical factors, social and economic status, ethnicity, genetics, metabolism and microbes to promoting healthy growth, body composition and wellbeing in the children.

Detailed Description

Not available

Recruitment & Eligibility

Status
ENROLLING_BY_INVITATION
Sex
All
Target Recruitment
510
Inclusion Criteria

All children still participating in the NiPPeR study at 6-8 years.

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Exclusion Criteria

Known significant congenital anomaly or genetic condition that influences child growth or neurodevelopment.

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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Prior Intervention - enhanced micronutrients, probiotics, myo-inositol in preconception & pregnancyIntervention (Study Nutritional Drink)NiPPeR Study intervention drink containing a mix of micronutrients, probiotics and myo-inositol given to the mother during preconception \& pregnancy
Prior Control - standard micronutrients in preconception & pregnancyControl (Standard Nutritional Drink)NiPPeR Study control nutritional drink containing a mix of micronutrients given to the mother during preconception \& pregnancy
Primary Outcome Measures
NameTimeMethod
Child Body Mass Index (kg/m2), derived from weight and heightUp to age 8 years, from periconception to age 8 years

Child size and body composition

Strengths & Difficulties Questionnaire score (no units)Up to age 8 years, from periconception to age 8 years

Child neurobehavioural functioning

Intellectual function (WASI-II IQ [4 sub-test scores], higher score is a higher IQ)Up to age 8 years, from periconception to age 8 years

Child cognition

Dimensional Change Card Sort Test (raw & T-scores)Up to age 8 years, from periconception to age 8 years

Child executive functioning - NIH Toolbox Cognition Battery component

Child insulin resistance (HOMA2-IR [no units], higher value is greater insulin resistance)Up to age 8 years, from periconception to age 8 years

Child insulin resistance

Flanker Inhibitory Control & Attention Test (raw & T-scores)Up to age 8 years, from periconception to age 8 years

Child executive functioning - NIH Toolbox Cognition Battery Component

Child Behaviour Check List score (no units)Up to age 8 years, from periconception to age 8 years

Child neurobehavioural functioning

Secondary Outcome Measures
NameTimeMethod
Change in lean mass (kg)Up to age 8 years, from periconception to age 8 years

Lean mass gain

Body bone mass (kg, % and indices)Up to age 8 years, from periconception to age 8 years

Child body composition - bone mass

Child height (cm)Up to age 8 years, from periconception to age 8 years

Child height

Change in weight (kg)Up to age 8 years, from periconception to age 8 years

Weight gain

Body lean mass (kg, % and indices)Up to age 8 years, from periconception to age 8 years

Child body composition - lean mass

Change in body mass index (z-score & percentile)Up to age 8 years, from periconception to age 8 years

Change in BMI

Child mid-upper arm circumference (cm)Up to age 8 years, from periconception to age 8 years

Child arm circumference

Change in waist circumference (z-score)Up to age 8 years, from periconception to age 8 years

Adiposity gain

Heart rate response to step test (beats per minute)Up to age 8 years, from periconception to age 8 years

Cardiovascular fitness and function

Diagnosed medical conditions or disorders ascertained by questionnaire, including child medical conditions, infections, gastrointestinal, cardiovascular, respiratory, dermatological, neuropsychological, mental and musculoskeletal health and wellbeingUp to age 8 years, from periconception to age 8 years

Child health and wellbeing

Parental Feeding Practices QuestionnaireUp to age 8 years, from periconception to age 8 years

Parental feeding practices

Hair metabolome panel concentrations (pmol/L)Up to age 8 years, from periconception to age 8 years

Biomarkers of health and wellbeing measured in hair samples

Body fat mass (kg, % and indices)Up to age 8 years, from periconception to age 8 years

Obesity and body composition

Change in mid-upper circumference (z-score)Up to age 8 years, from periconception to age 8 years

Change in soft tissue mass

Child blood pressure (mm Hg)Up to age 8 years, from periconception to age 8 years

Cardiovascular fitness and function

Child resting heart rate (beats per minute)Up to age 8 years, from periconception to age 8 years

Cardiovascular fitness and function

Child Eating Behaviour QuestionnaireUp to age 8 years, from periconception to age 8 years

Child eating behaviours

Children's Sleep Habits QuestionnaireUp to age 8 years, from periconception to age 8 years

Child sleep patterns

Children's actigraphy measurements of activity behaviours [minutes/day]Up to age 8 years, from periconception to age 8 years

Child physical activity, sleep and sedentary behaviours

Skin pricks test results to allergens [mm]Up to age 8 years, from periconception to age 8 years

Child atopic sensitisation

Change in body fat mass (kg)Up to age 8 years, from periconception to age 8 years

Adiposity gain

Change in bone mass (kg)Up to age 8 years, from periconception to age 8 years

Bone mass gain

Height growth (cm)Up to age 8 years, from periconception to age 8 years

Linear growth

Child minutes to return to resting heart rate (minutes)Up to age 8 years, from periconception to age 8 years

Cardiovascular fitness and function

Stool metagenomic profile (abundance)Up to age 8 years, from periconception to age 8 years

Child gut microbiota composition

Skin metagenomic profile (abundance)Up to age 8 years, from periconception to age 8 years

Child skin microbiota composition

Buccal transcriptomic profile (relative expression)Up to age 8 years, from periconception to age 8 years

Child buccal cell transcriptomic profile

Plasma high-sensitivity C-reactive protein concentrations (mmol/L)Up to age 8 years, from periconception to age 8 years

Child's inflammatory marker status

Child waist circumference (cm)Up to age 8 years, from periconception to age 8 years

Child waist circumference

Dietary patterns ascertained by frequency questionnairesUp to age 8 years, from periconception to age 8 years

Child diet

Child asthma, wheezing illnesses, eczema, rhinitis and food allergy by clinical examination & questionnairesUp to age 8 years, from periconception to age 8 years

Child atopic and allergic wellbeing and disorders

Urine micronutrient concentrations (nmol/L)Up to age 8 years, from periconception to age 8 years

Child micronutrient status

Blood trace element concentrations (pmol/L)Up to age 8 years, from periconception to age 8 years

Child circulating trace element profile

Blood molecular profile (% methylation)Up to age 8 years, from periconception to age 8 years

Child blood epigenetic profile

Plasma glucose concentration (mmol/L)Up to age 8 years, from periconception to age 8 years

Child's plasma glycemia

Blood metabolome panel concentrations (pmol/L)Up to age 8 years, from periconception to age 8 years

Child's blood metabolomic status

Child Movement Behaviour QuestionnaireUp to age 8 years, from periconception to age 8 years

Child activity behaviours

Urine trace element concentrations (pmol/L)Up to age 8 years, from periconception to age 8 years

Child trace element status

Genetic variation (DNA sequence analysis)Up to age 8 years, from periconception to age 8 years

Genetic variant analysis

Circulating adipokine measurements (nmol/L)Up to age 8 years, from periconception to age 8 years

Child adipokine profile

Circulating inflammatory marker measurement (nmol/L)Up to age 8 years, from periconception to age 8 years

Child inflammatory factor profile

Urine metabolome panel concentrations (pmol/L)Up to age 8 years, from periconception to age 8 years

Child's urine metabolomic status

Urine measurement of environmental pollutant exposure (pmol/L)Up to age 8 years, from periconception to age 8 years

Urine exposome pollutant profile

Hair measurement of environmental pollutant exposure (pmol/L)Up to age 8 years, from periconception to age 8 years

Hair exposome pollutant profile

Blood micronutrient concentrations (nmol/L)Up to age 8 years, from periconception to age 8 years

Child circulating micronutrient profile

Stool metatranscriptomic profile (relative activity)Up to age 8 years, from periconception to age 8 years

Child gut microbiota activity profile

Skin metatranscriptomic profile (relative activity)Up to age 8 years, from periconception to age 8 years

Child skin microbiota activity profile

Skin metabolomic profile (pmol/L)Up to age 8 years, from periconception to age 8 years

Child skin microbiota metabolite profile

Buccal molecular profile (% methylation)Up to age 8 years, from periconception to age 8 years

Child buccal cell epigenetic profile

Blood transcriptomic profile (relative expression)Up to age 8 years, from periconception to age 8 years

Child blood transcriptomic profile

Plasma lipid concentrations (mmol/L)Up to age 8 years, from periconception to age 8 years

Child's metabolic profile - lipid concentrations

Blood measurement of environmental pollutant exposure (ng/gm lipid)Up to age 8 years, from periconception to age 8 years

Blood exposome pollutant profile

Stool metabolomic profile (pmol/L)Up to age 8 years, from periconception to age 8 years

Child gut microbiota metabolite profile

Circulating chemocytokine measurements (nmol/L)Up to age 8 years, from periconception to age 8 years

Child chemocytokine profile

Trial Locations

Locations (3)

University Hospital Southampton

🇬🇧

Southampton, Hampshire, United Kingdom

The University of Auckland

🇳🇿

Auckland, New Zealand

National University Hospital

🇸🇬

Singapore, Singapore

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