Influence of human plasma versus serum on transfection efficiency of mRNA nanoparticles
- Conditions
- Geen: het betreft fundamenteel onderzoek naar de werking van PepFect14-mRNA nanodeeltjes-
- Registration Number
- NL-OMON51048
- Lead Sponsor
- Radboud Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 3
Inclusion Criteria
Healthy indivuals, 18-60 years of age, bodyweight: above 50 kg. Being able and
willing to complete the informed consent process.
Exclusion Criteria
Known to have a condition which results in more than minimal risk upon blood
drawing, e.g. hemophilia.
A medical condition which requires active medical intervention or monitoring to
avert serious danger to the individual's health or well-being.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main parameters are the Cy5-mRNA uptake and EGFP expression after<br /><br>transfection of cells with PF14-EGFP mRNA or PF14 with Cy5-labeled EGFP mRNA,<br /><br>in the presence of plasma or serum. Cy5 and EGFP levels will be measured by<br /><br>confocal laser scanning microscopy (CLSM).</p><br>
- Secondary Outcome Measures
Name Time Method <p>N.a.</p><br>