Effect of Ceftazidime-Avibactam Against Carbapenemase-Positive Klebsiella Pneumoniae Causing Health Care-Associated Infections
Overview
- Phase
- Not Applicable
- Status
- Not yet recruiting
- Sponsor
- Sohag University
- Enrollment
- 100
- Locations
- 1
- Primary Endpoint
- 1 ISOLATION OF KLEB. by culture on macConkey agar
Overview
Brief Summary
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
- analysisThe resistance pattern of the isolates will be detected by disc diffusion method.
- phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Detailed Description
Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention.
Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
- analysisThe resistance pattern of the isolates will be detected by disc diffusion method.
- phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Study Design
- Study Type
- Observational
- Observational Model
- Case Control
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.-
Exclusion Criteria
- •Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
Arms & Interventions
Group 1 patients with klebsiella pneumoniae infection
Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.
Intervention: . - Genotypic detection of carbapenemase genes by conventional PCR. (Genetic)
Group 2 patients without klebsiella pneumoniae
Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
Intervention: . - Genotypic detection of carbapenemase genes by conventional PCR. (Genetic)
Outcomes
Primary Outcomes
1 ISOLATION OF KLEB. by culture on macConkey agar
Time Frame: 6 months from January to may
Antibiotic sensitivity testing of different antibiotics according to clsi 25
Time Frame: 6 months from January to may
Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae
Time Frame: 6 months from January to may
Detection of resistance gene in isolates using conventional pcr
Time Frame: 6 months from June to December
Detection of some virulence genes using conventional pcr
Time Frame: 6 months from June to December
Secondary Outcomes
No secondary outcomes reported
Investigators
Walaa ismail Marzouk
Demonstrator at medical microbiology and immunology department faculty of medicine so
Sohag University