Effect of Ceftazidime-Avibactam Against Carbapenemase-Positive Klebsiella Pneumoniae Causing Health Care-Associated Infections
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- 1 ISOLATION OF KLEB. by culture on macConkey agar
研究概览
简要总结
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
- analysisThe resistance pattern of the isolates will be detected by disc diffusion method.
- phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
详细描述
Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention.
Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
- analysisThe resistance pattern of the isolates will be detected by disc diffusion method.
- phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.-
排除标准
- •Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
研究组 & 干预措施
Group 1 patients with klebsiella pneumoniae infection
Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.
干预措施: . - Genotypic detection of carbapenemase genes by conventional PCR. (Genetic)
Group 2 patients without klebsiella pneumoniae
Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
干预措施: . - Genotypic detection of carbapenemase genes by conventional PCR. (Genetic)
结局指标
主要结局
1 ISOLATION OF KLEB. by culture on macConkey agar
时间窗: 6 months from January to may
Antibiotic sensitivity testing of different antibiotics according to clsi 25
时间窗: 6 months from January to may
Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae
时间窗: 6 months from January to may
Detection of resistance gene in isolates using conventional pcr
时间窗: 6 months from June to December
Detection of some virulence genes using conventional pcr
时间窗: 6 months from June to December
次要结局
未报告次要终点
研究者
Walaa ismail Marzouk
Demonstrator at medical microbiology and immunology department faculty of medicine so
Sohag University