Evaluation of In vitro Anti-psoriatic Activity of a Novel Polyherbal UNIMFormulation by Multiparametric Analysis

Psoriasis has been recognized as a chronic inflammatory disease of the skin characterized by an accelerated rate of keratinocyte proliferation, alteration in dermal angiogenesis and increased production of proinflammatory cytokines.In conventional system of medicine topical and oral steroids with or without phototherapy are commonly prescribed for the management of psoriasis. But long term use of these medicines may invite several unwanted adverse side effects like hypertension, hypercholesterolemia, etc. So there is a dire need to develop new formulation which can provide long term relief without major side effects. Unani system of medicine is concerned Psoriasis is described under the heading of Taqashshure Jild caused by deposition of Khilte ghaleez (Abnormal Sauda) in skin, and treated by various modes of treatments. , but their mechanism of action at cellular level is still not clear. Thus there is considerable research interest to look in anti-proliferative, pro-apoptotic, anti-inflammatoryand anti-angiogenic activity. identification of determine mechanism of action of UNIM formulation at cellular level using in vitro model on the in traditional medicine which are being used   since centauries.other anti-inflammatory agents especially those which are being used in traditional medicine since centauries.Unani system of medicine has rich treasure of therapeutically active anti-inflammatory products and extensive patient beneficial clinical experience on many chronic diseases. However there is lack of any mechanistic study and their effect at cellular level, which can be best explained by using the in-vitro cell model. Herbs, such as Rheum  palmatum L, Rehmannia glutinosa Libosch and Lonicera japonica have been used for the treatment of skin inflammatory diseases including psoriasis and atopic dermatitis, but their mechanism of action at cellular level is still not clear . Unani system of medicine has therapeutically active anti-inflammatory Products such UNIM 401, a novel polyherbal concoction of these 3 herbs in the ratio of 1:1:3, which demonstrated efficacious effects in the treatment of psoriasis. These findings encouraged us to determine mechanism of action of UNIM 401 at cellular level using in vitro models and assessing effect on the key markers of psoriasis.

The study focuses on the complex interactions between genetic predisposition and epigenetic alterations involved in pathogenesis of psoriasis . Objective of the study is to evaluate the effect  of serum NO, IL-22, TNF-α, IFN-γ, IL-6, sPLA2,VEGF levels in Psoriasis patients and follow-ups  taking  UNIM 401 formulation  compared to healthy controls