Rhabdoid Tumors Cellular Architecture by Single-cell Analyses (InnovRT-2)

Completed

• Conditions: ATRT
• Interventions: Biological: analyzes in singles cells

• Registration Number: NCT04987476
• Lead Sponsor: Assistance Publique - Hôpitaux de Paris

Brief Summary

The aim is to describe at the cellular level the heterogeneity of rhabdoid tumors, and identify how this diversity influences resistance to treatment.

Detailed Description

Rhabdoid tumors are aggressive cancers of infancy. Although frequently chemosensitive at the very beginning, they very often show resistance, suggesting some intra-tumor diversity or plasticity. Moreover, morphological analysis of rhadoid tumors often reveal some variety in tumor cell shapes and immunohistochemical profiling. Altogether, this suggests some intra-tumor heterogeneity, that has been scarcely studied so far.

This project aims to describe the intra-tumor heterogeneity by single-cell sequencing approaches.

After surgical resection, fresh tumor samples will be dissected and analysed using the 10X Chromium technology. Briefly, cells will be grouped in clusters according to their gene expression signalling, and each cluster will be defined in comparison with all other ones. Differential analyses will allow identifying the main characteristic of each cell sub-population. Potential filiations between clusters will be analysed using classical algorithms. The study will be performed on 10 to 15 tumor samples.

Study Timeline

• Study First Submitted: 2021-07-31
• Study First Submitted QC: 2021-07-31
• Study First Posted: 2021-08-03
• Study Start: 2022-03-31
• Status Verified: 2025-02
• Primary Completion: 2025-02-21
• Study Completion: 2025-02-21
• Last Update Submitted: 2025-03-10
• Last Update Posted: 2025-03-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

• Aged 0-17 years old
• having a rhabdoid tumor according to clinical and radiological features Or likely having a rhabdoid tumor according to clinical and radiological features
• surgical resection in standard care
• sufficient material for both diagnosis and experimental procedures
• parents' agreement

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
ATRT	analyzes in singles cells	Children affected by an ATRT at time of surgical resection

Primary Outcome Measures

Name	Time	Method
Intra-tumor heterogeneity	Inclusion	Identification of cell clusters - mean expression (RPKM unit) of gene set signtaures in each bioinformatically defined cluster

Secondary Outcome Measures

Name	Time	Method
Naming of potential targetable gene	Inclusion	Mean expression (RPKM unit) of targetable genes (defined by the existence of adequate pharmacological inhibitor) in each tumor sample and clusters
Identification of cytotoxic cells	Inclusion	Conducting therapeutic biological functional tests
Cell clusters recurrent from one sample to the other	Inclusion	Fraction/percentage of cell clusters defined by primary outcome that are specific to each tumor and those that are shared by several or all samples

Trial Locations

Locations (1)

Institut Curie; 🇫🇷 Paris, France