Hétérogénéité Des Tumeurs Rhabdoides : Approches en " Cellules Uniques " Pour Identifier Des Cibles thérapeutiques
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Intra-tumor heterogeneity
研究概览
简要总结
The aim is to describe at the cellular level the heterogeneity of rhabdoid tumors, and identify how this diversity influences resistance to treatment.
详细描述
Rhabdoid tumors are aggressive cancers of infancy. Although frequently chemosensitive at the very beginning, they very often show resistance, suggesting some intra-tumor diversity or plasticity. Moreover, morphological analysis of rhadoid tumors often reveal some variety in tumor cell shapes and immunohistochemical profiling. Altogether, this suggests some intra-tumor heterogeneity, that has been scarcely studied so far.
This project aims to describe the intra-tumor heterogeneity by single-cell sequencing approaches.
After surgical resection, fresh tumor samples will be dissected and analysed using the 10X Chromium technology. Briefly, cells will be grouped in clusters according to their gene expression signalling, and each cluster will be defined in comparison with all other ones. Differential analyses will allow identifying the main characteristic of each cell sub-population. Potential filiations between clusters will be analysed using classical algorithms. The study will be performed on 10 to 15 tumor samples.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- — 至 17 Years（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Aged 0-17 years old
- •having a rhabdoid tumor according to clinical and radiological features Or likely having a rhabdoid tumor according to clinical and radiological features
- •surgical resection in standard care
- •sufficient material for both diagnosis and experimental procedures
- •parents' agreement
排除标准
- 未提供
研究组 & 干预措施
ATRT
Children affected by an ATRT at time of surgical resection
干预措施: analyzes in singles cells (Biological)
结局指标
主要结局
Intra-tumor heterogeneity
时间窗: Inclusion
Identification of cell clusters - mean expression (RPKM unit) of gene set signtaures in each bioinformatically defined cluster
次要结局
- Naming of potential targetable gene(Inclusion)
- Identification of cytotoxic cells(Inclusion)
- Cell clusters recurrent from one sample to the other(Inclusion)