Comparison Across Multiple Types of Sleep Deprivation
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Sleep Deprivation
- Sponsor
- Civil Aerospace Medical Institute
- Enrollment
- 59
- Locations
- 2
- Primary Endpoint
- Gene expression changes associated with different sleep patterns.
- Status
- Completed
- Last Updated
- 2 years ago
Overview
Brief Summary
This study is designed to assess neurobehavioral performance, as well as genetic and other physiological changes associated with variations in timing and quantity of sleep.
Detailed Description
During a 1.5 week inpatient laboratory protocol, subjects will undergo one of four parallel sleep conditions. Subjects will be randomly assigned to each condition group. During their time in the laboratory, biological samples (e.g., blood) will be collected for genetic and other analyses, and sleep quantity and quality will be monitored. Additionally responses on neurobehavioral tests and surveys will be monitored for differences among the groups.
Investigators
Eligibility Criteria
Inclusion Criteria
- •BMI 18.5-29.9, with consideration of BMIs 30-34.9.
Exclusion Criteria
- •Medication use, with potential consideration for contraceptives.
- •Color blindness
- •Recent nightwork, shiftwork, or travel across greater than two time zones
- •Pregnant or within 6 months post-partum
Outcomes
Primary Outcomes
Gene expression changes associated with different sleep patterns.
Time Frame: Samples for gene expression will be collected at frequent intervals over 10 days in a sleep laboratory. Subjects will be in a time-free environment, and not told sampling frequency.
Levels of expression of all genes may be compared across time and among study arms with different sleep patterns using RNA-Seq and differential expression analysis with generalized linear models. Patterns may be sought with and potential relevance to sleep, circadian rhythms, and/or neurobehavioral performance.
Secondary Outcomes
- Neurobehavioral performance changes associated with different sleep patterns.(Neurobehavioral assays may be conducted at frequent intervals over 10 days in a sleep laboratory. Subjects will be in a time-free environment, and not told assay frequency.)
- DNA genetic variants associated with sleep and/or neurobehavioral performance.(Approximately two oral samples may be taken for DNA analyses during the 10 days in a sleep laboratory.)