Study of Autologous Stem Cell Transplantation for Patients With Ischemic Stroke

Phase 1

Completed

• Conditions: Infarction, Middle Cerebral Artery

• Registration Number: NCT00473057
• Lead Sponsor: Universidade Federal do Rio de Janeiro

Brief Summary

This is a open label study to assess the safety of autologous bone marrow transplantation in patients with a ischemic stroke in the middle cerebral artery territory within 90 days from symptoms onset.

Detailed Description

Objective: The purpose of this study is to evaluate the safety and feasibility of intra-arterial and intravenous injection of autologous bone marrow mononuclear cells in patients in the acute and sub-acute phase (\> 3 and \< 90 days after symptoms onset) of ischemic cerebral infarct in the middle cerebral artery territory.

Methods: 15 patients with cerebral infarct within the middle cerebral artery territory will be enrolled in this prospective, nonrandomized, open-labeled study. Up to 10 patients will receive up to 500x 10 6 autologous bone marrow cells injected intra-arterially into the middle cerebral artery through percutaneous approach. The procedure is monitored by Transcranial Doppler (TCD) and electroencephalogram (EEG). Other 5 patients will receive up to 500x 10 6 autologous bone marrow cells injected intravenously. Changes in neurological deficits and improvements in functions will be evaluated at baseline and at regular intervals during follow-up ( 4 months). EEG and neuroimaging exams will be performed at baseline and at regular intervals during the follow-up period.

Study Timeline

• Study Start: 2005-12
• Study First Submitted: 2007-05-10
• Study First Submitted QC: 2007-05-10
• Study First Posted: 2007-05-14
• Status Verified: 2011-05
• Primary Completion: 2011-05
• Study Completion: 2011-05
• Last Update Submitted: 2011-05-18
• Last Update Posted: 2011-05-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• clinical diagnosis of ischemic stroke lasting for more than 3 hours and less than 90 days from symptoms onset
• neuroimages exams showing ischemic cerebral infarct in the middle cerebral artery territory
• age between 18 and 75 years old
• NIHSS between 4 and 20
• spontaneous re-canalization of the middle cerebral artery documented by TCD or MRI
• signed informed consent

Exclusion Criteria

• difficult in obtaining vascular access for percutaneous procedures
• vascular impossibility to reach the middle cerebral artery through percutaneous approach
• severe carotid stenosis( >70%, by Doppler) related to the severe stroke
• neurological worsening (>4 points in the NIHSS ) due to edema or intracerebral hemorrhage
• primary hematological disease
• neurodegenerative disorder
• previous stroke with mRS > 2
• intracardiac thrombosis
• auto-imune disorders
• osteopathies that could increase the risk of bone marrow harvesting procedure
• thrombophilias
• liver failure
• chronic renal failure (creatinine > 2mg/ml)
• life support dependence
• lacunar stroke
• pregnancy
• history of neoplasia or other comorbidity that could impact patient's short-term survival
• any condition that in the judgment of the investigator would place the patient under undue risk

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Primary Outcome Measures

Name	Time	Method
Absence of new neurological deficits during the procedure and/or in the 4 months follow-up.	4 months

Secondary Outcome Measures

Name	Time	Method
Improvement of neurological deficits	4 months
Improvement in the neuroimaging exams	4 months

Trial Locations

Locations (1)

Hospital Universitário Clementino Fraga Filho/UFRJ; 🇧🇷 Rio de Janeiro, RJ, Brazil