Substrate Metabolism During 2 Hours of Moderate Intensity Exercise Following Ingestion of Ethanol in Man
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 8
- 试验地点
- 2
- 主要终点
- Change in muscle glycogen content
研究概览
简要总结
This study will investigate how ethanol (pure alcohol) influences carbohydrate and fat metabolism during prolonged, moderate intensity exercise. Participants will perform two bouts of cycling exercise with or without prior ingestion of ethanol, in a randomised order, separated by one week.
详细描述
Very little research has investigated the influence of ethanol on metabolism during prolonged exercise. Evidence suggests that delivery of fat to the working muscles during exercise may be limited with ethanol and therefore this may shift the relative proportions of energy derived from muscle stores of carbohydrate as a result (Jorfeldt & Juhlin-Dannfelt, 1976).
The current study aims to investigate how a small-moderate dose of ethanol influences where energy is derived from during a prolonged bout of moderate intensity cycling exercise.
Participants will visit the lab on three occasions, once for preliminary measurements of fitness and body composition, and twice for the experimental bouts of exercise. Body composition will be assessed using a dual-energy x-ray absorptiometry (DEXA) scan, which will measure lean mass, fat mass, and bone mineral density. Participants will then perform an incremental maximal oxygen uptake test to determine intensity for the experimental bouts of exercise.
The two experimental bouts of exercise include cycling on an exercise bike for 2 hours at 55% of their maximal oxygen uptake (i.e. fitness) following 1 hours rest. This will be performed under two conditions: with and without ethanol ingestion. Pre and post exercise muscle biopsies will be collected to assess muscle metabolism, with regular blood samples and expired breath samples being collected to further investigate fuel delivery and use by the working tissues. The experimental bouts of exercise will be performed 1 week apart in a randomised order.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Habitual alcohol consumer (average 1-14 units per week)
- •BMI 18.5 - 29.9 kg/m2
- •Be able to attend the laboratory and willing to participate in necessary protocols
- •Be willing to undertake the durations of the exercise protocol (2 hours) ]
- •Have the capacity and willingness to provide informed consent (oral and written)
排除标准
- •Non-or excessive alcohol drinkers (0 or >14 units per week)
- •History of substance abuse
- •Habitual/previously habitual smokers
- •BMI >30.0 kg/m2
- •Experience contraindications to the administration of the proposed anaesthetic (Lidocaine Hydrochloride)
研究组 & 干预措施
No Ethanol
Participants will ingest a volume matched beverage of water only.
Ethanol
Participants will ingest ethanol (in the form of 40% ethanol) at an ingestion rate of 0.1 grams/kg lean body mass/hour in a solution with water.
干预措施: Ethanol (Dietary Supplement)
结局指标
主要结局
Change in muscle glycogen content
时间窗: Change in muscle glycogen content over 2 hours of moderate intensity cycling exercise
Muscle biopsy and subsequent biochemical analysis
Blood non-esterified fatty acid (NEFA) concentration
时间窗: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Venous blood samples will be taken and biochemically analysed for NEFA concentration.
Blood glucose concentration
时间窗: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Venous blood samples will be taken and biochemically analysed for blood glucose concentration.
次要结局
- Blood lactate concentration(Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.)
- Carbohydrate Oxidation(Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour)
- Fat Oxidation(Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour)
- Blood ethanol concentration(Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.)
研究者
James Betts
Principal Investigator
University of Bath