Influence of NaCl intake on Microcirculation and Immune system

Conditions: Salt, Sodium, Microcirculation, Immune system, Zout, Natrium, Microcirculatie, Immuunsysteem

Registration Number: NL-OMON28966

Lead Sponsor: Academic Medical Center

Brief Summary: one

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: Not specified

Target Recruitment: 18

Inclusion Criteria

Male between 18 and 40 years of age

- Healthy, as determined by a responsible and experienced physician, based on a medical
evaluation including medical history, physical examination (PE) and laboratory tests carried
out in the screening visit.

Exclusion Criteria

- An office blood pressure >130/85 mmHg

- A body mass index > 30 kg/m2

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Several primary endpoints are proposed. 1. Microcirculation A. To assess the effect of dietary sodium intake on capillary recruitment and capillary perfusion determined by capillary density, proportion of perfused density, microculatory flow index and tortuosity, assessed by SDF-imaging, nailfold capillaroscopy and retinal vascular imaging. B. To assess whether high sodium-induced changes in microcirculation can be restored by nitroglycerin, being a NO donor to the capillary vessel bed. 2. Immune system A. To assess whether different sodium intakes (high or low salt diet) will lead to changes in circulating T-lymphocyte subpopulations (e.g., Th17 cells).

Secondary Outcome Measures

Name	Time	Method
1. Microcirculation C. To assess if microcirculatory changes in response to dietary sodium are related to macrocirculatory changes, displayed by measurement of central and peripheral blood pressure by use of continuous finger arterial pressure (FinAp) waveform registration with the semi-automatic device Nexfin® and by using radial pulse waveforms with the semi-automatic device Sphygmocor®. 3. Other A. To assess whether different sodium intakes will lead to changes in eNOS and RNA expression and sulfation of glycosaminoglycans (GAGs) of the skin.