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A Study Evaluating the Efficacy and Safety of ABP 959 Compared With Eculizumab in Adult Participants With PNH

Phase 3
Completed
Conditions
Paroxysmal Nocturnal Hemoglobinuria
Interventions
Registration Number
NCT03818607
Lead Sponsor
Amgen
Brief Summary

This is a randomized, double-blind, active-controlled phase 3 study of ABP 959 in participants with paroxysmal nocturnal hemoglobinuria.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
42
Inclusion Criteria
  • Men and women ≥ 18 years of age.
  • Historical diagnosis of PNH.
  • Administration of eculizumab for ≥ 6 months and currently receiving 900 mg of eculizumab.
  • Hemoglobin ≥ 9.0 g/dL for at least 6 weeks before randomization.
  • Lactate dehydrogenase < 1.5 × the upper limit of normal at screening.
  • Platelet count ≥ 50 × 10^9/L.
  • Absolute neutrophil count (ANC) ≥ 0.5 x 10^9/L (500/μL).
  • Participants must be vaccinated against Neisseria meningitidis.
  • Participants must sign an IRB/IEC-approved ICF before participation in any procedures.
Exclusion Criteria
  • Known or suspected hereditary complement deficiency.
  • Clinically significant cardiovascular disease (including myocardial infarction, unstable angina, symptomatic congestive heart failure [New York Heart Association ≥ Class III], serious uncontrolled cardiac arrhythmia), peripheral vascular disease, cerebrovascular accident, or transient ischemic attack in the previous 6 months.
  • Evidence of acute thrombosis (liver Doppler ultrasound of hepatic and portal veins).
  • Known to be positive for human immunodeficiency virus.
  • Woman who is pregnant or breastfeeding.
  • Participant is currently enrolled in or has not yet completed at least 30 days since ending other investigational device or drug study(s), or participant is receiving other investigational agent(s).
  • Participant has known sensitivity to any of the products to be administered during the study, including mammalian cell-derived drug products.
  • History of meningococcal infection.
  • Presence or suspicion of active bacterial infection, or recurrent bacterial infection.
  • History of bone marrow transplantation.
  • Red blood cell transfusion required within 12 weeks before randomization.
  • Participant experienced ≥ 2 breakthrough events, (ie, signs and symptoms of intravascular hemolysis, that require dose and/or schedule adjustments of eculizumab) in the previous 12 months before screening.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
T (ABP 959) / R (eculizumab)ABP 959ABP 959 for 52 weeks in Period 1 followed by eculizumab for 26 weeks in Period 2
R (eculizumab) / T (ABP 959)ABP 959Eculizumab for 52 weeks in Period 1 followed by ABP 959 for 26 weeks in Period 2
R (eculizumab) / T (ABP 959)EculizumabEculizumab for 52 weeks in Period 1 followed by ABP 959 for 26 weeks in Period 2
T (ABP 959) / R (eculizumab)EculizumabABP 959 for 52 weeks in Period 1 followed by eculizumab for 26 weeks in Period 2
Primary Outcome Measures
NameTimeMethod
LDH Level at Week 27 (Parallel Comparison)Week 27

The primary analysis for the parallel comparison was hemolysis as measured by LDH at Week 27 by initial treatment received (Period 1).

Time-adjusted Area Under the Effect Curve (AUEC) of LDH (Crossover Comparison Per Assigned Treatment)From Week 13 to Week 27, from Week 39 to Week 53, and from Week 65 to Week 79

The primary analysis for the crossover comparison was hemolysis, as measured by the time-adjusted AUEC of LDH, according to treatment assigned during each of the 14-week assessments during Periods 1 and 2.

Secondary Outcome Measures
NameTimeMethod
Mean LDH Levels by Visit up to Week 79Baseline, Week 3, Week 7, Week 13, Week 15, Week 19, Week 25, Week 27, Week 29, Week 33, Week 39, Week 41, Week 43, Week 45, Week 47, Week 49, Week 51, Week 53, Week 55, Week 59, Week 65, Week 67, Week 69, Week 71, Week 73, Week 75, Week 77, and Week 79

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Total Complement (50% Total Hemolytic Complement Activity [CH50])Baseline, Week 27, Week 39, Week 53, Week 65, and Week 79

Total complement (%) was measured in serum using an assay method and compared the total hemolytic complement activity to the lower limit of the normal human reference (LLN) of 58 U/mL for all CH50 values. The percent of LLN of CH50 at each time point was calculated as mean CH50 results/LLN x 100%.

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Total and Unbound Pharmacokinetics (PK) Area Under the Curve (AUC) of ABP 959 and Eculizumab From Week 13 to Week 15 (Period 1)PK samples were collected predose and immediately postdose Week 13, 7 days post the Week 13 dose (Week 14), and predose at Week 15

The total and unbound PK concentration AUC values from Week 13 to Week 15 in Period 1 are presented by actual treatment received.

Degree of HemoglobinuriaBaseline, Week 27, Week 39, Week 53, Week 65, and Week 79

The degree of hemoglobinuria was categorized as negative, trace, small, moderate, and large based on the analysis of urine samples collected from each participant at the specified time points.

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Haptoglobin LevelsBaseline, Week 27, Week 39, Week 53, Week 65, and Week 79

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Bilirubin LevelsBaseline, Week 27, Week 39, Week 53, Week 65, and Week 79

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Number of Packed RBC Units Transfused Per MonthBaseline to End of Study (up to Week 79)

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Total Hemoglobin LevelsBaseline, Week 27, Week 39, Week 53, Week 65, and Week 79

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Serum-free Hemoglobin LevelsBaseline, Week 27, Week 39, Week 53, Week 65, and Week 79

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Mean Percentage of Type III ErythrocytesBaseline, Week 27, Week 39, Week 53, Week 65 and Week 79

As a measure of hemolysis the mean percentage of Type III erythrocytes was measured at the specified timepoints.

Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

LDH Levels at Week 53 and Week 79Week 53 (first week of Period 2) and Week 79 (last week of Period 2)

The analysis of the crossover comparison of hemolysis, as measured by LDH at Week 53 and Week 79.

Total and Unbound Trough Serum Concentrations of ABP 959 and EculizumabPK samples were collected predose at the prespecified timepoints: baseline, Week 3, Week 7, Week 13, Week 15, Week 19, Week 27, Week 33, Week 39, Week 45, Week 51, Week 53, Week 55, Week 59, Week 65, Week 71, Week 77, and Week 79

The total and unbound serum trough concentrations are presented by treatment sequence received for the prespecified time points. Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Number of Participants With Treatment-Emergent Adverse Events (TEAEs)Day 1 to End of Study (up to Week 79)

TEAEs are defined as any adverse event (AE) that began or increased in severity or frequency at or after the time of first treatment up to end of study (up to Week 79). A treatment-emergent serious adverse event (SAE) was a TEAE that met at least 1 of the following criteria: was fatal, life-threatening, required or prolonged inpatient hospitalization, resulted in persistent or significant disability/incapacity, was a congenital anomaly/birth defect, or was another medically important serious event.

The treatment-emergent events of interest (EOI) prespecified for this study included serious infections (meningococcus aspergillus, and other serious infections/sepsis), and infusion reactions.

Number of Participants With Antidrug Antibodies (ADAs)Blood samples for ADA assessments were taken predose at baseline, Week 3, Week 7, Week 13, Week 19, Week 25, Week 27, Week 33, Week 39, Week 45, Week 51, Week 53, Week 55, Week 59, Week 65, Week 71, Week 77 and Week 79.

Any samples that tested positive for binding antibodies were also tested for neutralizing antibodies. Treatment boosted ADAs were defined as a positive immunoassay result at baseline and at least 1 postbaseline immunoassay result that was ≥ 4 times the magnitude of the baseline result. Baseline was defined as the last non-missing assessment taken prior to the first dose of IP.

Trial Locations

Locations (24)

Saint James's Hospital

🇮🇪

Dublin, Ireland

Fakultní Nemocnice Ostrava

🇨🇿

Ostrava-Poruba, Czechia

Fakultní Nemocnice Brno

🇨🇿

Brno, Jihormoravsky KRAJ, Czechia

King's College Hospital NHS Foundation Trust

🇬🇧

London, England, United Kingdom

Karolinska Universitetssjukhuset - Huddinge

🇸🇪

Stockholm, Sweden

Children's Healthcare of Atlanta at Egleston

🇺🇸

Atlanta, Georgia, United States

Mersin Universitesi Tip Fakultesi

🇹🇷

Mersin, Turkey

Fakultní Nemocnice Olomouc

🇨🇿

Olomouc, Czechia

Hospital Universitario La Fe

🇪🇸

Valencia, Spain

Univerzitetni klinični center Ljubljana

🇸🇮

Ljubljana, Slovenia

Azienda Ospedaliera San Gerardo di Monza

🇮🇹

Monza, Monza Brianza, Italy

Hospital Universitario de Salamanca

🇪🇸

Salamanca, Spain

The Leeds Teaching Hospitals NHS Trust

🇬🇧

Leeds, England, United Kingdom

Ege Universitesi Hastanesi - Sağlık Uygulama ve Araştırma Merkezi

🇹🇷

Bornova, Izmir, Turkey

Fondazione Policlinico Universitario Agostino Gemelli

🇮🇹

Roma, Italy

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori

🇮🇹

Meldola, Forli-cesena, Italy

Azienda Ospedaliera S. Croce e Carle Cuneo

🇮🇹

Cuneo, Italy

Azienda USL della Romagna

🇮🇹

Ravenna, Italy

Keski-Suomen keskussairaala Jyväskylä

🇫🇮

Jyväskylä, Finland

Päijät-Häme Central Hospital

🇫🇮

Lahti, Finland

Hôpital Privé Sévigné

🇫🇷

Cesson-Sevigne, Bretagne, France

Instituto Português de Oncologia do Porto Francisco Gentil

🇵🇹

Porto, Portugal

Oslo University Hospital - Rikshospitalet

🇳🇴

Oslo, Norway

Radboud Universitair Medisch Centrum

🇳🇱

Nijmegen, Gelderland, Netherlands

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