Effect of Statins on Epigenetic Reprogramming of Monocytes in Patients With Elevated Levels of LDL
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 45
- 试验地点
- 1
- 主要终点
- Cytokine production capacity
研究概览
简要总结
The innate immune system plays a pivotal role in the development and progression of atherosclerosis. Recently, it was reported that monocytes can develop a long-lasting immunological memory after stimulation with various microorganisms, which has been termed 'trained innate immunity'. This memory is induced by epigenetic reprogramming, in particular trimethylation of lysine 4 at histone 3 (H3K4me3).
In this study, the investigators aim to investigate the immunophenotype of circulating monocytes in patients with elevated LDL cholesterol levels and the effect of statins on this phenotype.
详细描述
The innate immune system plays a pivotal role in the development and progression of atherosclerosis. Recently, it was reported that monocytes can develop a long-lasting immunological memory after stimulation with various microorganisms, which has been termed 'trained innate immunity'. This memory is induced by epigenetic reprogramming, in particular trimethylation of lysine 4 at histone 3 (H3K4me3).
The main objective is to study whether circulating monocytes of patients with elevated levels of LDL have a phenotype of trained innate immunity compared to control patients. Subsequently the investigators will study the effect of treatment with statins on the increased inflammatory response and epigenetic changes of the innate immune system.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Aged 18 years or older
- •Elevated LDL levels (>4.9 mmol/l)
- •Decision made by treating physician to initiate statin treatment
排除标准
- •previous cardiovascular events.
研究组 & 干预措施
High-LDL
patients with LDL cholesterol levels of >4.9 mmo/l, who have not been treated with statins in the past years, and who have an indication for treatment with statins.
干预措施: statin treatment (Drug)
结局指标
主要结局
Cytokine production capacity
时间窗: baseline
Cytokine production capacity of PBMC's at baseline in patients with elevated and normal cholesterol levels
次要结局
- Epigenetic modifications in the promoter regions of the pro-inflammatory cytokines in monocytes.(Three months after start of statin treatment)
- Cytokine production capacity(Three months after start of statin treatment)