Interactions Between Fibre and Polyphenols on Colonic Phenolic Acid Production and Biomarkers of Health ( 6 Weeks)

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Other: Tomato, onion and lovage soup (TOL); Other: TOL soup with inulin

• Registration Number: NCT03840746
• Lead Sponsor: University of Glasgow

Brief Summary

The investigators have studied the acute interactions of polyphenols and NDC in a single meal in a human study containing tomatoes, lovage \& onions, with and without inulin. The investigators now wish to study the longer-term interactions of these foods over 6 weeks as well as their impact on biomarkers of human health, the gut microbiome, human proteome and markers of risk of chronic disease. This may allow better targeted evaluation of foods which influence health through the gut microbiome.

Detailed Description

Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols.

Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids over a 6 week feeding trial and impact on biomarkers of cardiometabolic health.

Study Timeline

• Study Start: 2018-07-11
• Study First Submitted: 2019-01-18
• Study First Submitted QC: 2019-02-11
• Study First Posted: 2019-02-15
• Primary Completion: 2019-07-30
• Study Completion: 2019-09-30
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-24
• Last Update Posted: 2023-03-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 90

Inclusion Criteria

• Generally healthy
• BMI>=25
• Not on antibiotics within 3 months of starting of study
• No medication likely to influence gut function, colonic bacterial activity.

Exclusion Criteria

• Antibiotics
• Pregnancy
• lactation

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Tomato, onion and lovage soup (TOL)	Tomato, onion and lovage soup (TOL)	One tin of soup containing Tomato onion and lovage daily for 6 weeks
TOL soup with inulin	TOL soup with inulin	One tin of Tomato, onion and lovage soup with added inulin for 6 weeks

Primary Outcome Measures

Name	Time	Method
Change from baseline of total amount of phenolic acids (umoles/24 hours) in urine (3 wk)	3 weeks	Profile and amounts of phenolic acids in urine derived from the colonic bacterial metabolism of polyphenols
Change from baseline of total amount of phenolic acids (umoles/24 hours) in urine (6 wk)	6 weeks	Profile and amounts of phenolic acids in urine derived from the colonic bacterial metabolism of polyphenols

Trial Locations

Locations (1)

University of Glasgow; 🇬🇧 Glasgow, Scotland, United Kingdom