Acute Elevation of Non-esterified Fatty Acids on Endothelial Function and Insulin Sensitivity: A Comparison of Saturated and Long Chain n-3 Polyunsaturated Fatty Acids During the Postprandial Phase
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 59
- 试验地点
- 1
- 主要终点
- Flow-mediated dilatation
研究概览
简要总结
Experimental elevation of non-esterified fatty acids (NEFA) impairs endothelial function and insulin sensitivity but the impact of NEFA composition is unknown.
The objective was to test the effect of acute elevation of NEFA enriched with either saturated fatty acids (SFA) or SFA with long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) on postprandial vascular function measured via flow-mediated dilatation (FMD), laser Doppler iontophoresis (LDI) and digital volume pulse (DVP), followed by a hyperinsulinaemic-euglycaemic clamp as a measure of whole body insulin sensitivity.
详细描述
To investigate potential diet-gene interactions, potential subjects (n=370) were prospectively genotyped for the eNOS Glu298Asp polymorphism, of which 35 were Asp298 and 150 were Glu298 homozygotes. Three subjects in the Asp298 group were unable to participate, two were unsuitable according to selection criteria and one subject subsequently withdrew from the study. Subjects homozygous for Asp298 (n=29) and Glu298 (n=30) were therefore selected, balanced for gender, age and BMI.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant)
排除标准
- •Raised fasting blood lipids
- •Taking excessive fish oil supplements (>1g EPA/DHA per day)
- •Taking medication known to influence blood lipids, blood pressure or blood clotting
研究组 & 干预措施
SFA
Oral dose of palmitic acid (SFA) given as a chocolate-flavoured drink every 30 min (0-390 min) with a continuous infusion of heparin (60-390 min).
干预措施: Absence or presence of fish oil (Dietary Supplement)
SFA + LC n-3 PUFA
Oral dose of palmitic acid and DHA-rich fish oil (SFA + LC n-3 PUFA) given as a chocolate-flavoured drink every 30 min (0-390 min) together with a continuous infusion of heparin (60-390 min).
干预措施: Absence or presence of fish oil (Dietary Supplement)
结局指标
主要结局
Flow-mediated dilatation
时间窗: Change from 240 min to 390 min
Flow-mediated dilatation
时间窗: Change from baseline to 240 min
次要结局
- Laser Doppler iontophoresis(Change from 240 min to 390 min)
- Circulating endothelial markers(Change from 240 min to 390 min)
- NEFA composition(Change from baseline to 240 min)
- Circulating endothelial function markers(Change from baseline to 240 min)
- Insulin sensitivity(390 min)
- Digital volume pulse(Change from 240 min to 390 min)
- Digital volume pulse(Change from baseline to 240 min)
- Laser Doppler iontophoresis(Change from baseline to 240 min)