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The Tunnel Technique and Free Gingival Graft in Gingival Recessions Treatment

Not Applicable
Recruiting
Conditions
Gingival Recession
Interventions
Procedure: Free gingival graft
Procedure: Tunnel technique
Procedure: Laterally closed tunnel technique
Procedure: Gingival graft - donor area
Registration Number
NCT05270161
Lead Sponsor
Universidade Federal de Alfenas
Brief Summary

The present study aims to compare the tunnel technique/laterally closed tunnel technique and the FGG technique in the treatment of isolate/multiple GR in the anterior region of the mandible. Furthermore, it is proposed to evaluate the effect of a gel based on hyaluronic acid and green tea on the healing of the donor area after the removal of FGG from the palate. This work is expected to contribute to the still uncertain literature on the effectiveness of the tunnel technique as an alternative to the use of FGG in the treatment of multiple GR in the mandible.

Detailed Description

Free gingival graft (FGG) is a simple technique considered the most effective procedure to obtain gingival augmentation in sites with a minimal amount of keratinized gingiva. However, the abscence of chromatic integration and unsatisfactory texture of the graft after healing prevent its use in areas with high aesthetic demand. In this context, the tunnel technique (multiple gingival recessions \[GR\]) and the laterally closed tunnel technique (isolated GRs in the mandible) have been proposed as an alternative to the use of FGG in the treatment of GRs in the mandible. The present study aims to compare the tunnel technique/laterally closed tunnel technique and the FGG technique in the treatment of isolate/multiple GR in the anterior region of the mandible. Furthermore, it is proposed to evaluate the effect of a gel based on hyaluronic acid and green tea on the healing of the donor area after the removal of FGG from the palate. Sixty participants presenting isolate/multiple Miller Class I, II, or III GR \[Recession type (RT) 1 or RT2\] in the anterior region of the mandible will be included in the present study. Regarding the treatment of GR, participants will be divided into four groups: (1) Test Group 1 (T1) (n=15): multiple GR in the anterior region of the mandible treated with the tunnel technique; (2) Test Group 2 (T2) (n=15): isolate GR in the anterior region of the mandible treated with the laterally closed tunnel technique; (3) Control Group 1 (C1) (n=15): multiple GRs in the anterior region of the mandible will be treated using the FGG technique and (4) Control Group 2 (C2) (n=15): isolate GRs in the anterior region of the mandible will be treated using the FGG technique. Regarding the treatment of the donor area, the same participants will be divided into two groups: (1) Gel Group (G) (n=30): the donor area on the palate will be treated using hyaluronic acid gel and green tea applied by the participant 3 times a day for 7 days; (2) Clot Group (CO) (n=30): No material will be placed in the donor area, only the clot will be kept in position by means of sutures. Periodontal clinical parameters will be evaluated in the receiving area at the beginning of the study and after 1, 3, 6 and 12 months postoperatively. In the donor area, the percentage of wound closure, the epithelialized surface area and the color of the palatine mucosa will be evaluated in both groups after 3, 7, 15 and 30 days. The visual analogue scale (VAS) of pain (donor and recipient area) and aesthetic (recipient area) will be used to assess the participant's perception of the treatment used.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Age between 18 and 60 years old;
  • Present isolated (≥4mm) or multiple GR in the anterior region of the mandible (tooth 33 to 43) Miller Class I, II or III (87) (or Recession type (RT) 1 or RT2);
  • Systemically healthy with no contraindications for periodontal surgery;
  • Probing depth of less than 3mm at the sites involved;
  • Selected teeth properly positioned, free from endodontic treatment, any type of restorative treatment, caries or non-carious cervical lesion
Exclusion Criteria
  • Participants who use tobacco or other types of drugs;
  • Participants submitted to periodontal treatment in the last 6 months;
  • Pregnant participants;
  • Participants who did not accept to return to follow-ups;
  • Participants who used antibiotics, corticosteroids, chemotherapy, immunomodulators or others that modify the periodontal therapy results during the last 6 months.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Control Group 2 (C2)Free gingival graftIsolate gingival recessions in the anterior region of the mandible will be treated using the free gingival graft technique.
Test Group 1 (T1)Tunnel techniqueMultiple gingival recessions in the anterior region of the mandible treated with the tunnel technique.
Test Group 2 (T2)Laterally closed tunnel techniqueIsolate gingival recessions in the anterior region of the mandible treated with the laterally closed tunnel technique.
Control Group 1 (C1)Free gingival graftMultiple gingival recessions in the anterior region of the mandible will be treated using the free gingival graft technique.
Test Group 1 (T1)Gingival graft - donor areaMultiple gingival recessions in the anterior region of the mandible treated with the tunnel technique.
Test Group 2 (T2)Gingival graft - donor areaIsolate gingival recessions in the anterior region of the mandible treated with the laterally closed tunnel technique.
Control Group 1 (C1)Gingival graft - donor areaMultiple gingival recessions in the anterior region of the mandible will be treated using the free gingival graft technique.
Control Group 2 (C2)Gingival graft - donor areaIsolate gingival recessions in the anterior region of the mandible will be treated using the free gingival graft technique.
Gel Group (G)Gingival graft - donor areaThe donor area on the palate will be treated using hyaluronic acid gel and green tea applied by the participant 3 times a day for 7 days.
Clot Group (CO)Gingival graft - donor areaNo material will be placed in the donor area, only the clot will be kept in position by means of sutures.
Primary Outcome Measures
NameTimeMethod
Recession depthChange from baseline recession depth at 1, 3, 6 and 12 months

Distance between cemento-enamel junction to free gingival margin.

Secondary Outcome Measures
NameTimeMethod
Recession widthChange from baseline recession width at 1, 3, 6 and 12 months

Mesio-distal extent of gingival recession

Gingival margin positionChange from baseline gingival margin position at 1, 3, 6 and 12 months

Distance from a reference point performed on the customized stent to the gingival margin.

Graft thickness after removalBaseline

Graft thickness will be assessed immediately after removal using a endodontic file in the middle of the graft. The distance between the marker and the tip of the file will be determined using a digital caliper.

Width of keratinized tissue in graftChange from immediate postoperative width of keratinized tissue in graft at 1, 3, 6 and 12 months

Mesio-distal dimension of the graft in the most coronal portion of the graft using the periodontal probe.

Percentage of wound reduction by photographic imageChange from 3 days percentage of wound reduction by photographic image at 7, 15 and 30 days

The millimeter periodontal probe will be positioned next to the wound at the time of obtaining the photos to calibrate the image analysis software that will be used to calculate the total area of the wound in donor region.

Percentage of Wound Reduction by clinical measurementChange from 3 days percentage of wound reduction by clinical measurement at 7, 15 and 30 days

The height and width of the wound will be determined using the periodontal probe.

Palatal mucosa colorChange from 3 days palatal mucosa color at 7, 15 and 30 days

The color of the palatal mucosa will be evaluated by the visual analogue scale (VAS) in comparison with the color of the adjacent and opposite mucosa. In this evaluation, on a horizontal scale, 0 points will represent lack of color correspondence, 50 points will represent average correspondence and 100 points will represent excellent color correspondence with the adjacent tissues.

Probing depthChange from baseline probing depth at 1, 3, 6 and 12 months

Distance between free gingival margin to the bottom of the sulcus.

Clinical attachment levelChange from baseline clinical attachment level at 1, 3, 6 and 12 months

Distance between cemento-enamel junction to the bottom of the sulcus.

Keratinized tissue widthChange from baseline keratinized tissue width at 1, 3, 6 and 12 months

Distance between the most apical point on free gingival margin to the mucogingival junction.

Gingival thicknessChange from baseline gingival thickness at 6 and 12 months

The measurements were registered at the mid-buccal aspect of the tooth using a endodontic file. The distance between the marker and the tip of the file will be determined using a digital caliper.

Postoperative complications7 days

Presence of bleeding, swelling, infection and necrosis in the donor area will be recorded

Graft thickness immediately before sutureBaseline

The thickness of the graft immediately before suturing will be evaluated after de-epithelialization (in the T group) and the removal of adipose tissue when present in both groups in the middle of the graft. The distance between the marker and the tip of the file will be determined using a digital caliper.

Graft contractionChange from immediate postoperative graft contraction at 1, 3, 6 and 12 months

Graft areas will be calculated by multiplying the height and width of keratinized tissue in graft.

Landry indexChange from 3 days landry index at 7 and 30 days

Classify the degree of repair based on erythema, presence of granulation tissue, bleeding/suppuration, and epithelialization in the recipient area. A score of 1 to 5 is given, where 1 is associated with a very poor/inadequate repair process and 5 is excellent.

Visual Analogue Scale (VAS) for painChange from 3 days VAS scale for pain at 7, 12 and 30 days

The perception of pain will be recorded using the VAS scale at the operated sites. Participants will record the level of pain in the recipient and donor areas after the procedure on a horizontal scale, where the left endpoint will mean no pain (0), the midpoint of mean pain (50 ) and the right outcome severe pain (100).

Presence or absence of dentin sensitivityChange from 1 month dentin sensitivity at 3, 6 and 12 months

Determined from the participants' responses regarding the presence or absence of sensitivity in the cervical sites. For this, it will be asked if the participant has or not dentin sensitivity after a cold or air stimulus.

Height of keratinized tissue in graftChange from immediate postoperative height of keratinized tissue in graft at 1, 3, 6 and 12 months

Apico-coronal dimension of the graft measured at the stent mark using the periodontal probe.

Epithelized surface areaChange from 3 days epithelized surface area at 7, 15 and 30 days

The epithelialization analysis will be performed using 3% oxygen peroxide to observe the presence of blistering in the wound. The absence of epithelialization will be characterized as blistering after contact of oxygen peroxide with the wound. This parameter will be noted dichotomously (presence/absence of bubbles), being noted as yes or no, respectively, in the participant's form.

Root coverage esthetic score (RES)12 months

The RES system evaluates five variables of the recipient area including: gingival margin level, marginal tissue contour, soft tissue texture, JMG alignment and gingival color. Zero, 3 or 6 points will be used to assess the position of the gingival margin, while a score of 0 or 1 point will be used for each of the other variables. The ideal aesthetic score will be 10.

Visual Analogue Scale (VAS) for esthetics12 months

Participants will be asked to express their overall satisfaction with treatment outcomes on a VAS scale, where the left endpoint will mean not at all satisfied (0), the midpoint reasonably satisfied (50) and the right endpoint extremely satisfied. satisfied (100).

Analgesics consumption7 days

The consumption of analgesics by the participants in the first 7 postoperative days will also be recorded.

Trial Locations

Locations (1)

Universidade Federal de Alfenas

🇧🇷

Alfenas, Minas Gerais, Brazil

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