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Blended Oil Beneficial for Blood Lipids Management

Not Applicable
Conditions
Hyperlipidemias
Interventions
Other: Refined olive oil (ROO)
Other: Blended Oil 1 (BO1)
Other: Blended Oil 2 (BO2)
Registration Number
NCT03964857
Lead Sponsor
JeyaKumar Henry
Brief Summary

To investigate the effects of two types of oil blends on blood lipid profile, glucose homeostasis, body composition, gut microflora and other markers of cardiometabolic disease risk in Chinese men and women with borderline hyperlipidemia

Detailed Description

The primary aims of this study is to evaluate the effects of consumption of two separate blends of cooking oil, containing rice bran, flaxseed and sesame oils, for a period of 8 weeks, on blood total cholesterol, total triglycerides, low-density and high-density lipoprotein cholesterol and metabolism in borderline hyperlipidaemic, 50 to 70 years old, Chinese, male and postmenopausal female, as compared to refined olive oil. The secondary aims of the study is to evaluate the effect of the two separate blends of cooking oil on markers of cardiometabolic diseases risk (including body composition, glucose homeostasis, markers of oxidative stress and chronic inflammation) and changes in gut microbiota composition and function.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
120
Inclusion Criteria
  • Male and post-menopausal female,
  • Chinese ethnicity
  • Age ≥50 and ≤70 years
  • LDL cholesterol (measured) ≥3.06 mmol/l and ≤4.51 mmol/l
Exclusion Criteria
  • Body Mass Index (BMI) > 27.5 kg/m2.
  • Having allergies / intolerances to the foods in the meal accompaniments of the study
  • Having undergone bariatric surgery
  • Having fasting glucose ≥7.0 mmol/l
  • Having heart, kidney (nephrotic syndrome), blood or thyroid dysfunctions
  • History of chronic liver diseases including NAFLD, liver cirrhosis or hepatic fibrosis or having history of HIV, Hep-B or Hep-C virus
  • Having prescription medication or Traditional Chinese Medicine or supplements known to affect blood lipid metabolism, in the opinion of the study investigators, in the last two weeks prior to screening
  • Having diabetes or chronic gastrointestinal disorders
  • Following any special diets (for medical or aesthetic reasons)
  • Smoking
  • Having had diarrhea in the last 3 months
  • Having taken antibiotics in the last 3 months

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Refined olive oil (ROO)Refined olive oil (ROO)Control
Blended Oil 1Blended Oil 1 (BO1)BO1, propriety blend of cooking oil containing rice bran, flaxseed and sesame oil blended at proportions different from 'Blended Oil 2
Blended Oil 2Blended Oil 2 (BO2)BO2, propriety blend of cooking oil containing rice bran, flaxseed and sesame oil blended at proportions different from 'Blended Oil 1
Primary Outcome Measures
NameTimeMethod
Change from baseline serum total cholesterol8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline serum low density lipoprotein cholesterol8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline serum total high density lipoprotein cholesterol8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline serum total triglycerides8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Secondary Outcome Measures
NameTimeMethod
Change from baseline of serum insulin8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline of serum glucose8 weeks measured every 2 weeks

Blood will be collected in plain serum tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in plasma C-reactive protein8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in bone mineral density8 weeks

measured using DEXA (Dual-Energy X-ray Absorptiometry)

Change from baseline in plasma oxidised low density lipoproteinFor 8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in plasma glycated albumin8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in plasma Tumor necrosis factor-α8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in blood pressure8 weeks measured every 2 weeks

Measured using Omron blood pressure monitor after an overnight fast in a seated posture, at rest. Blood pressure measured for a minimum of 3 times and the average of the final 2 readings were taken, ensuring that they were within 10 mmHg of each other.

Change from baseline in plasma fructosamine8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in plasma Interleukin-68 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in body fat composition percentage8 weeks

measured using DEXA (Dual-Energy X-ray Absorptiometry)

Change from baseline in segmental % body fat8 weeks measured every 2 weeks

Body impedance assessments (BIA)

Change from baseline in plasma Interleukin-1β8 weeks measured every 2 weeks

Blood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collectionBlood will be collected in EDTA tubes after an overnight fast, centrifuged and frozen within 2 hours of collection

Change from baseline in gut microflora function8 weeks measured on days14 and 56

Assessed using gut metagenome and metatranscriptome profiling

Change from baseline in Body weight8 weeks measured every 2 weeks

Body impedance assessments (BIA)

Change from baseline in gut microflora composition8 weeks measured on days14 and 56

Assessed using gut metagenome and metatranscriptome profiling

Change in body circumference measures8 weeks measured every 2 weeks

Using measuring tape

Trial Locations

Locations (1)

Clinical Nutrition Research Centre

🇸🇬

Singapore, Singapore

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