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Gut Microbiota, SCFAs and Glucolipid Metabolism in Pregnant Women With Abnormal Fetal Size and Their Newborns

Conditions
Fetal Macrosomia
Fetal Growth Restriction
Glucolipid Metabolism
Gut Microbiota
Interventions
Other: test gut microbiota, SCFAs and glucolipid metabolism and insulin sensitivity
Registration Number
NCT04399434
Lead Sponsor
Second Affiliated Hospital of Wenzhou Medical University
Brief Summary

Abnormal fetal size includes fetal growth restriction and fetal macrosomia. Onset is closely related to maternal nutrition metabolism. The specific correlation and mechanism is unclear, and there are no effective measures for early diagnosis and treatment. Previous study found that maternal gut microbiota participates in the material metabolism throughout the pregnancy. Insulin sensitivity in pregnant women, and intrauterine environment under abnormal blood glucose and lipid metabolism are important for the gut microbiota of newborns and even they grow up. However, changes in gut microbiota are the cause of the disease or the outcome is not yet clear. Short chain fatty acids (SCFAs) are produced from soluble dietary fibers in the diet by colon bacteriolysis. Studies have found that gut microbiota can regulate insulin sensitivity and glucose and lipid metabolism disorders through SCFAs. Therefore, this research group uses the gut microbiota as a new idea to studythe relationship of gut microbiota characteristics and level's change of SCFAs with glucolipid metabolism and insulin sensitivity in pregnant women with abnormal fetal size and their newborns through 16S-rRNA high-throughput sequencing, pyrosequencing, and gas chromatography-mass spectrometry, so we can reveal the role of gut microbiota in the pathogenesis of abnormal fetal size and explore targeted rational dietary adjustment and SCFAs reconstruction of gut microbiota to improve maternal and neonatal pregnancy outcomes.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
120
Inclusion Criteria
  • Singleton pregnancy
  • Term pregnancy with the gestational age of 37-40 weeks
Exclusion Criteria
  • Maternal systemic diseases (hypertension disorders, immunological diseases) or pregnant complications (polyhydramnios, oligohydramnios, diabetes mellitus, intrahepatic cholestasis of pregnancy)
  • Delivery before 37 weeks or after 40 weeks
  • Neonates had major congenital malformations (congenital anal atresia, congenital biliary atresia, congenital heart disease)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
normal pregnancytest gut microbiota, SCFAs and glucolipid metabolism and insulin sensitivitypregnancy with a normal fetal size
fetal growth restrictiontest gut microbiota, SCFAs and glucolipid metabolism and insulin sensitivityfetal birth weight is below two standard deviations of the average weight for the same gestational age, or below the 10th percentile of normal weight for the same age
fetal macrosomiatest gut microbiota, SCFAs and glucolipid metabolism and insulin sensitivityfetal birth weight ≥ 4000g
Primary Outcome Measures
NameTimeMethod
Gut Microbiota and SCFAs in mothers and their newbornsStool samples from pregnant women with 37-40 gestational week and newborns within 3 days after birth

Collect the DNA of fecal flora to test the diversity and abundance of gut microbiota as well as short chain fatty acids in Stool samples

glucolipid metabolism and insulin sensitivity in pregnant womenBlood samples from pregnant women with 37-40 gestational week

glucolipid metabolism and insulin sensitivity in pregnant women with normal and abnormal fetal size

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

department of obstetrics of Second Affiliated Hospital of Wenzhou Medical University

🇨🇳

Wenzhou, Zhejiang, China

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