?ffects of the relative expression of genes of sperm DNA methyltransferase on global methylation in patients with severe sperm abnormalities before and after folic acid uptake
Not Applicable
- Conditions
- -Oligospermia.
- Registration Number
- IRCT201507285261N2
- Lead Sponsor
- Yazd research and clinical center for infertility
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- Male
- Target Recruitment
- 90
Inclusion Criteria
Inclusion criteria includes severe sperm parameters and protamine disrupted, age less than 40, avoiding the use of drugs or alcohol, and varicocele
Exclusion criteria: Unwillingness to continue during study,Diabetes,Blood Hypertension, obesity
Exclusion Criteria
Not provided
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Sperm parameters including count, motility, morphology. Timepoint: Two months after the intervention. Method of measurement: Microscopic examination of sperm.;Relative gene expression of methyltransferase. Timepoint: Two months after the intervention. Method of measurement: PCR Real time.;Phosphate group condensation. Timepoint: Two months after the intervention. Method of measurement: Toloidine Blue.;Protamin deficiency. Timepoint: Two months after the intervention. Method of measurement: CMA3.
- Secondary Outcome Measures
Name Time Method Flatulence, insomnia. Timepoint: Two month after intervention. Method of measurement: Questionnaire.