Effect of anticoagulants or procoagulants on thrombin generation in PPP, PRP and whole blood

• Conditions: bleeding; coagulation disorder; coagulopathy; thrombosis; 10064477; 10014523

• Registration Number: NL-OMON39676
• Lead Sponsor: niversiteit Maastricht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 29 April 2024

Recruitment & Eligibility

• Status: Pending
• Sex: Not specified
• Target Recruitment: 175

Inclusion Criteria

Healthy men and women between 18 and 65 years

Exclusion Criteria

- Use of a drug that interferes with coagulation (e.g. oral anticoagulants, oral contraceptives, oral antiplatelet drugs).
- A history of bleeding or thrombosis

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Thrombin generation will be determined in PPP, PRP and whole blood with or<br /><br>without flow. The data that will be collected through these experiments will be<br /><br>analysed with the use of 4 parameters: endogenous thrombin potential (ETP),<br /><br>peak height, time to peak and lag time. The thrombin generation experiments<br /><br>will be performed in the absence and presence of anticoagulant agents or agents<br /><br>that will possibly affect thrombin formation. If necessary, clotting factor<br /><br>determinations will be performed on the stored PPP, these can be antigen<br /><br>determinations or functional determinations.<br /><br><br /><br>Control experiments need to be carried out in order to check the quality of the<br /><br>blood/plasma by using the standard procedures of the lab: e.g. cell count, flow<br /><br>cytometry, aggregometry, clotting*</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>NA</p><br>