Identification of respiratory viruses among SARI patients using real-time PCR and third generation sequencing for development of molecular respiratory panel
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 200
- 试验地点
- 1
- 主要终点
- Understanding the seasonality and prevalence of various respiratory viruses causing SARI in
研究概览
简要总结
The study aims to estimate the prevalence of common respiratory viruses among SARI patients in Manipal, by establishing a third-generation sequencing platform for direct sequencing of clinical samples and developing a molecular respiratory panel for rapid virus identification. The methodology includes collecting 200 throat/nasopharyngeal swab samples from Kasturba hospital, Manipal and, performing nucleic acid extraction and real-time PCR, and sequencing using Oxford Nanopore technology for both PCR-positive and negative samples. The resulting data will be used in the development of a molecular respiratory virus panel
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 0.00 Day(s) 至 99.00 Year(s)（—）
- 性别
- All
入选标准
- •Patients will be recruited as per the WHO SARI case definition.
- •"An acute respiratory infection patients with the history of fever (≥ 38°C) and cough within the last 10 days and are hospitalized" and regardless of age, gender or any other underlying health condition.
排除标准
- •Patients who do not meet the WHO SARI case definition will be excluded from the study.
结局指标
主要结局
Understanding the seasonality and prevalence of various respiratory viruses causing SARI in
时间窗: Within 10 days of the onset of the illness
Manipal can help in designing better public health intervention strategies
时间窗: Within 10 days of the onset of the illness
次要结局
- Not applicable(Not applicable)
研究者
Dr Anitha J
Manipal Institute of Virology, Manipal Academy of Higher Education