The effects of glycogen availability on the skeletal muscle molecular response after concurrent exercise

Completed

• Conditions: Effect van inspanning met lage glycogeenbeschikbaarheid op de moleculaire respons; Not applicable

• Registration Number: NL-OMON42458
• Lead Sponsor: Wageningen Universiteit

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 23 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 14

Inclusion Criteria

• Male gender between 18 and 30 years of age
• BMI between 18.5 - 25 kg/m2
• Participating in regular exercise training (at least 1 exercise session, and a maximum of 3 exercise session per week)
• Used to different types of exercise (endurance, resistance and/or concurrent)
• Willing to give blood samples
• Willing to give muscle biopsies
• No use of antibiotics during the study or in the past month
• No drug use
• Suitable veins for blood withdrawal
• Registered by a general-practitioner
• Consumption of alcohol beverages is less than 21 per week
• No blood donor during the study
• No vegetarian and willing to eat meat during the experimental days
• Able to be present and participate at both test days and pre testing moments
• Not employed, or intern, or working on thesis at the department of Human Nutrition at Wageningen University
• Not participating in another scientific study (except EetMeetWeet)
• Able to participate during the experimental days en pre tests
• Able to speak Dutch

Exclusion Criteria

• Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, gluten intolerance)
• Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg
• Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate)
• Use of antithrombotic therapy (marcoumar, sintromitis).
• Diagnosed diabetes mellitus type 1 or 2
• (Chronic) injuries of the locomotor system that can interfere with the intervention

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- Phosphorylation of intramuscular proteins<br /><br><br /><br>- Changes in mRNA expression</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- A spectrum of post exercise myokines, cytokines and myogenic regulatory<br /><br>factors will be measured.<br /><br><br /><br>- Muscle glycogen<br /><br><br /><br>- Glucose, insulin, free fatty acids, cortisol, adrenalin and lactate<br /><br><br /><br>- Perceived exertion </p><br>