Effect of Anthocyanins Extracted From Purple Potatoes on Healthy Study Subjects' Postprandial Glycemia and Insulinemia
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Turku
- Enrollment
- 18
- Locations
- 1
- Primary Endpoint
- Plasma glucose concentration
Study Overview
Brief Summary
A randomized, single-blind cross-over clinical nutrition trial will be conducted. The effects of yellow-fleshed potatoes and yellow-fleshed potatoes supplemented with anthocyanins extracted from purple-fleshed potatoes on healthy men's postprandial glycemia and insulinemia will be compared. Additionally, the postprandial metabolism and metabolites, and the appearance and metabolism of the flavonoids and their metabolites is studied in plasma, urine and feces.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- Single (Investigator)
Eligibility Criteria
- Ages
- 18 Years to 45 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Age 18-45
- •No regular medication
- •No participation in a drug trial or blood donation within 2 months
- •Non-smoker
- •Signs informed consent
- •Body mass index 18.5-27 kg/m2
- •Fasting plasma cholesterol < 5.5 mmol/l
- •Fasting plasma triacylglycerols < 2.6 mmol/l
- •Fasting plasma glucose 4-6 mmol/l
- •blood pressure <140/80 mmHg
- •hemoglobin>130 g/l
- •Fasting plasma alanine amino transferase (ALAT) <60 U/l (normal liver function)
- •Fasting plasma thyroid-stimulating hormone (TSH) 0.4 - 4.5 mU/l (normal thyroid function)
- •Fasting plasma creatinine <118 µmol/l (normal kidney function)
Exclusion Criteria
- •Regular smoking
- •Alcohol abuse
- •Regular medication
Arms & Interventions
Anthocyanins and yellow potato
168 mg of anthocyanins extracted from purple-fleshed potatoes added to 350 g of steam-cooked mashed potatoes in water
Intervention: Dietary (Other)
Yellow potato
350 g of steam-cooked mashed potatoes in water
Intervention: Dietary (Other)
Outcomes
Primary Outcomes
Plasma glucose concentration
Time Frame: 0min, 20min, 40min, 60min, 90min, 120min, 180min, 240min
Plasma insulin concentration
Time Frame: 0min, 20min, 40min, 60min, 90min, 120min, 180min, 240min
Secondary Outcomes
- Concentrations and quality of metabolites and flavonoid color compounds and their metabolites in plasma (NMR and (U)HPLC-MS/MS measurements)(0min, 20min, 40min, 60min, 90min, 120min, 180min, 240min)
- Concentrations and quality of metabolites and flavonoid color compounds and their metabolites in urine (NMR and (U)HPLC-MS/MS measurements)(Before meal, and 0-4h, 4-8h, 8-16h, 16-24 h)
- Concentrations and quality of metabolites and flavonoid color compounds and their metabolites in feces (NMR and (U)HPLC-MS/MS measurements)(before and after meal)
- Area under the glucose concentration curve (AUC)(0min, 20min, 40min, 60min, 90min, 120min, 180min, 240min)
- Area under the insulin concentration curve (AUC)(0min, 20min, 40min, 60min, 90min, 120min, 180min, 240min)
Investigators
Kaisa Linderborg
Principal Investigator
University of Turku