Matrilysin Expression in Different Stages of Colorectal Tumors

Completed

• Conditions: Colorectal Cancer Stage IV; Colorectal Cancer Stage 0; Colorectal Cancer Stage I; Colorectal Cancer Stage II; Colorectal Cancer Stage III
• Interventions: Procedure: colonic or colorectal resection

• Registration Number: NCT01570452
• Lead Sponsor: University of Roma La Sapienza

Brief Summary

Matrix metalloproteinases (MMPs) have been shown to be involved in cancer biology. Significant expression of MMP-7 (matrilysin) in colorectal cancer is mainly associated with metastatic disease even though it is expressed in most tumor states. Our purpose is to analyse MMP-7 in bowel and lymph nodes of different tumor stages and to evaluate its expression as a potential biomarker of cancer disease in patients surgically treated for benign and malignant colorectal tumors. Tumoral tissue, lymph nodes and serum samples from recruited Patients plus serum samples from healthy volunteers are analysed for matrilysin expression by histology, immunohistochemistry, ELISA and Western blotting.

If Matrilysin increases with increasing dysplasia and cancer disease stage in tumor tissue as well as in the regional lymph nodes it might be used as a complement in investigating suspected locally advanced cancer.

Detailed Description

Not available

Study Timeline

• Study Start: 2005-09
• Primary Completion: 2008-09
• Study Completion: 2012-02
• Status Verified: 2012-03
• Study First Submitted: 2012-03-31
• Study First Submitted QC: 2012-04-03
• Last Update Submitted: 2012-04-03
• Study First Posted: 2012-04-04
• Last Update Posted: 2012-04-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 38

Inclusion Criteria

• benign colonic neoplasm not suitable for endoscopic treatment,
• malignant colonic neoplasm with indication to primary surgical treatment

Exclusion Criteria

• neo-adjuvant radiotherapy,
• chemo-radiotherapy,
• language problems and withdrawal of consent

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
healthy volunteers	colonic or colorectal resection	healthy subjects not affected by benign or malignant colonic disease
benign colonic tumor patients	colonic or colorectal resection	patients affected by benign colonic tumor such as adenoma
cancer patient I-II stage	colonic or colorectal resection	Patients affected by colonic cancer in I-II stage
cancer patients III-IV stage	colonic or colorectal resection	Patients affected by colonic cancer in III-IV stage

Trial Locations

Locations (1)

Departement of Surgery Pietro Valdoni; 🇮🇹 Rome, Italy