Corneal Epithelial Wound Healing Difference Between Human Autoserum and Cord Blood Serum
- Conditions
- Corneal Epithelial Wound Healing
- Registration Number
- NCT00681642
- Lead Sponsor
- National Taiwan University Hospital
- Brief Summary
Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 7
- Healthy pregnant women
- Cesarean section for labor
- Complete placenta with umbilical cord retained
- Healthy individual
- The retained placenta and umbilical cord were not complete
- Individual with anemia or other hematologic disorder unsuitable for blood donation
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Cell proliferation 1 week
- Secondary Outcome Measures
Name Time Method Cell migration 1 week
Trial Locations
- Locations (1)
National Taiwan University Hospital, department of Ophthalmology
🇨🇳Taipei, Taiwan