Effects of Milk Proteins on Whole Body Protein Synthesis and Ectopic Lipid Deposition During Sucrose Overfeeding in Healthy Male Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- whole body protein synthesis
研究概览
简要总结
This study aims at assessing the hypothesis that dietary protein content is a major factor regulating lean body mass gain and energy expenditure during overfeeding, and thus long term body weight gain
To test this hypothesis, healthy normal weight male and female subjects will before and after a 7-day hypercaloric, high-sucrose diet (+40% excess energy as sucrose) and either a low (5% total energy) or a high (20% total energy) protein intake. Each subject will be studied with both low- and high protein diets according to a randomized, crossover study. On each occasion the following measurements will be done in basal conditions (after 2-day isoenergetic, controled diet) and at the end of the 7-day overfeeding:
- whole-body protein turnover, oxidation and synthesis in fasting conditions and fed conditions (13C-labelled leucine)
- intrahepatic and intramuscular fat concentration (1H-Magnetic Resonance Spectroscopy)
- Energy metabolism in fasted and fed conditions (indirect calorimetry)
- plasma concentration of glucose, non-esterified fatty acids, total triglyceride, very-low density lipoprotein (VLDL)-triglyceride, insulin, glucagon, growth hormone (GH), insulin-like growth factor 1 (IGF1), insulin-like growth factor binding protein (IGFBP) 1,2 and 3 in fasting and fed conditions The effects of high-protein and low-protein sucrose overfeeding on whole body protein synthesis will be compared using two-way ANOVA; relationships between changes in whole body protein synthesis on one hand, and intrahepatic/intramuscular fat concentrations, total energy expenditure, and plasma concentration of metabolic variables on the other hand, will be evaluated by linear regression analysis
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •good apparent health
- •low physical activity
- •non-smokers
排除标准
- •vegans or following any special diet
- •consumption of > 3 dl/day sweetened beverages
- •changes in body > +/- 3Kg over the past 3 months
- •contra-indications to magnetic resonance spectroscopy
- •drug or substance abuse
- •consumption > 10g/day alcohol
- •lactose intolerance
研究组 & 干预措施
high protein
20% protein diet
干预措施: 7-day overfeeding (Other)
low protein
7.5% protein diet
干预措施: 7-day overfeeding (Other)
结局指标
主要结局
whole body protein synthesis
时间窗: after two days of a run-in, isocaloric low sucrose diet and after 7 days of a hypercaloric, high sucrose diet
Whole body protein synthesis, protein turnover, and protein oxidation will be calculated using 13C-labelled leucine infusion in both fasted and fed conditions
次要结局
- intrahepatic and intramuscular fat concentration(after two days of a run-in, isocaloric low sucrose diet and after 7 days of a hypercaloric, high sucrose diet)
- Energy expenditure(after two days of a run-in, isocaloric low sucrose diet and after 7 days of a hypercaloric, high sucrose diet)
- Metabolic variables in plasma samples(after two days of a run-in, isocaloric low sucrose diet and after 7 days of a hypercaloric, high sucrose diet)
研究者
Luc Tappy, MD
Professor of Physiology
University of Lausanne