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Mechanisms of SGLT2 Inhibition in Pediatric Steatotic Liver Disease

Phase 2
Recruiting
Conditions
Non-Alcoholic Fatty Liver Disease
Interventions
Drug: Placebo Oral Tablet
Registration Number
NCT06355310
Lead Sponsor
Ann & Robert H Lurie Children's Hospital of Chicago
Brief Summary

This study is a randomized, double-blind, placebo-controlled trial specifically designed to evaluate the preliminary feasibility, initial efficacy and safety of SGLT2 inhibitors for treating NAFLD in adolescents with obesity.

Detailed Description

The overall aim of this pilot study is to evaluate the feasibility and obtain a preliminary estimate of efficacy and safety of the SGLT2 inhibitor, empagliflozin, in adolescents with obesity (BMI-percentile ≥95th) who have MRI-confirmed NAFLD (hepatic fat fraction ≥ 5.5%) and have normal fasting glucose.

Participants will take empagliflozin, once daily, in the morning, with or without food, in addition to receiving lifestyle/behavioral counseling throughout the study.

The following data will be collected throughout the course of the study: Physical exam with tanner staging, safety and fasting labs, fasting blood draw (biomarkers), urine sample, 2-stage clamp (overnight Stay),Stable isotope tracers (overnight Stay), MRI scan (MRS-Liver), BMI/anthropometrics, urine pregnancy test for female participants, iDXA scan (body fat and bone density), arterial stiffness and blood pressure.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
40
Inclusion Criteria
  • For clinical referral to screening visit:
  • BMI >30 kg/m2 or >95th BMI-Percentile
  • Age 16 to <21 at baseline
  • Elevated alanine aminotransferase (ALT) more than twice the upper limit of normal by gender (≥ 44 U/L for girls, ≥ 50 U/L for boys)63 within 3 months prior to screening (used for historic ALT value) OR diagnosis of NAFLD from ultrasound, MRI, or participants with biopsy-proven NASH within 12 months of screening.
  • History of lifestyle modification to treat obesity or NAFLD.
  • Tanner stage >2
  • Normal fasting glucose (fasting blood glucose <100 mg/dL)

To be obtained at screening visit:

  • Confirmation of obesity;
  • Tanner stage 2,3,4 or 5;
  • Normal fasting glucose tolerance (fasting blood glucose <100 mg/dL);
  • If Screening ALT is used as inclusion criteria (if > 2x historic ALT value (historical value obtained clinically within 12 months of screening visit), repeated after 4 weeks [unable to randomize until completed]. If the repeat ALT is more than 50% increased or decreased over the screening ALT, a third ALT should be obtained. If a third ALT is not within 50% of the previous value, then the subject is ineligible but may be rescreened at a later date. If
  • ALT is not used:
  • An ultrasound will be done to diagnose NAFLD if the diagnosis has not previously been made by ultrasound, MRI or biopsy.
  • A MRI-derived HFF ≥ 5.5%
  • Willingness to adhere to lifestyle considerations throughout the study
Exclusion Criteria
  • •ALT > 250U/L at screening

    • History of significant alcohol intake or current use
    • Impaired fasting glucose (>100 mg/dL)
    • Diabetes (type 1 or 2)
    • Current or recent (<6 months prior to enrollment) use of weight loss medication(s)
    • Vitamin E supplementation or use of metformin
    • Previous bariatric surgery
    • Prior use of empagliflozin
    • Lower limb infection/ulceration within 3 months of screening
    • Metal or magnetic implants, devices or objects inside of or on the body, which are not MRI compatible
    • Structural and functional urogenital abnormalities, that predispose for urogenital infections
    • Recent initiation (<3 months prior to enrollment) of anti-hypertensive or lipid medication(s)
    • Major psychiatric disorder
    • Known hypothalamic or pituitary dysfunction
    • Current pregnancy or plans to become pregnant
    • Females unwilling to be tested for pregnancy
    • Females who are sexually active and not protects by an effective method of birth control (e.g. UID or medication or patch)
    • Tobacco use
    • Significant liver dysfunction (levels >5 times the upper limit of normal (ULN)):
    • ALT (ULN = 50 U/L)
    • AST (ULN = 48 U/L)
    • GGT (ULN = 48 U/L)
    • ALP (ULN = 115 U/L)
    • Platelets < 150,000 cells/mm3
    • Total bilirubin 1.3 mg/dL
    • INR 1.3
    • Albumin <3.2 g/dL
    • Gilbert's Syndrome
    • Any known causes of liver disease (except NAFLD and NASH)
    • Significant renal dysfunction (estimated glomerular filtration rate [eGFR] < 80 mL/min/1.73 m2),
    • Diagnosed monogenic obesity
    • History of cancer
    • Untreated thyroid disorder
    • History of decompensation events (ascites, variceal bleeding, hepatic encephalopathy, or hepatocellular carcinoma)
    • Current or recent (<6 months prior to enrollment) use of medication(s) associated with weight gain (e.g. atypical anti-psychotics).

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Study interventionEmpagliflozin 10 MGEmpagliflozin 10 mg will be taken daily
Control armPlacebo Oral TabletPlacebo oral tablet will be taken daily
Primary Outcome Measures
NameTimeMethod
Hepatic Fat26-Weeks

change in hepatic fat fraction (Hepatic fat will be measured by MRI via proton density fat fraction (PDFF)) from baseline (first measurement time point) to 26-weeks.

Secondary Outcome Measures
NameTimeMethod
Body mass index26-Weeks

* BMI

* body fat% (total and visceral fat)

* ALT

* biomarkers of NAFLD oCK-18 ototal and activated PAI-1 oIL-8

* biomarkers of bone health (additional safety outcome) oN-terminal collagen type I extension propeptide (PINP) oOsteocalcin oC-terminal cross-linking telopeptide of type I collagen (CTX)

* Systolic and diastolic blood pressure

* glycemic control (from oral glucose tolerance test)

* Insulin sensitivity (from oral glucose tolerance test) by the whole-body insulin sensitivity index

Trial Locations

Locations (1)

Ann & Robert H Lurie Children's Hospital of Chicago

🇺🇸

Chicago, Illinois, United States

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