Evaluation of the Outcomes of the Embryos Obtained From Different Follicle Sizes, and the miRNA Detection in the Spent Media of Their Blastocyst Culture
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Embryo Quality
- Sponsor
- Acibadem University
- Enrollment
- 55
- Locations
- 1
- Primary Endpoint
- Oocyte grading
- Status
- Recruiting
- Last Updated
- 2 years ago
Overview
Brief Summary
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.
Detailed Description
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.
Investigators
Nazli Albayrak
Resident, MD
Acibadem University
Eligibility Criteria
Inclusion Criteria
- •Female patients aged between 20-38 who are planned to have IVF treatment
- •On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm
- •Patient having BMI below 30
Exclusion Criteria
- •PCO patients
- •Patients having endometriosis
- •Patients having male factor with severe oligoazoospermia
Outcomes
Primary Outcomes
Oocyte grading
Time Frame: after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started
Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes
Day-3 Embryo Grading
Time Frame: Three days after the intracytoplasminc sperm injection(ICSI) procedure
Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes
Day-5 Embryo Grading
Time Frame: 5 days after the intracytoplasminc sperm injection(ICSI) procedure
Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes
beta hCG results of the transferred embryos
Time Frame: ten days after the embryo transfer
Clinical pregnancy outcomes
Secondary Outcomes
- micro RNA expression in culture medium(Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment)