Evaluation of the Outcomes of the Embryos Obtained From Different Follicle Sizes, and the miRNA Detection in the Spent Media of Their Blastocyst Culture
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 55
- 试验地点
- 1
- 主要终点
- Oocyte grading
研究概览
简要总结
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.
详细描述
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 38 Years(Adult)
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Female patients aged between 20-38 who are planned to have IVF treatment
- •On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm
- •Patient having BMI below 30
排除标准
- •PCO patients
- •Patients having endometriosis
- •Patients having male factor with severe oligoazoospermia
结局指标
主要结局
Oocyte grading
时间窗: after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started
Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes
Day-3 Embryo Grading
时间窗: Three days after the intracytoplasminc sperm injection(ICSI) procedure
Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes
Day-5 Embryo Grading
时间窗: 5 days after the intracytoplasminc sperm injection(ICSI) procedure
Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes
beta hCG results of the transferred embryos
时间窗: ten days after the embryo transfer
Clinical pregnancy outcomes
次要结局
- micro RNA expression in culture medium(Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment)
研究者
Nazli Albayrak
Resident, MD
Acibadem University
