Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture

Recruiting

• Conditions: Embryo Quality
• Interventions: Procedure: Oocyte pick-up

• Registration Number: NCT05947526
• Lead Sponsor: Acibadem University

Brief Summary

In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.

Detailed Description

In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.

Study Timeline

• Study First Submitted: 2022-11-23
• Study Start: 2023-06-20
• Status Verified: 2023-07
• Study First Submitted QC: 2023-07-09
• Last Update Submitted: 2023-07-09
• Study First Posted: 2023-07-17
• Last Update Posted: 2023-07-17
• Primary Completion: 2023-10-20
• Study Completion: 2023-12-10

Recruitment & Eligibility

• Status: RECRUITING
• Sex: Female
• Target Recruitment: 55

Inclusion Criteria

• Female patients aged between 20-38 who are planned to have IVF treatment
• On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm
• Patient having BMI below 30

Exclusion Criteria

• PCO patients
• Patients having endometriosis
• Patients having male factor with severe oligoazoospermia

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Women referring to infertility clinic for IVF treatment	Oocyte pick-up	Women who were referred to infertility clinic with complaint of not having baby go through IVF treatment, and having oocyte pick-up procedure with different sizes of ovarian follicles

Primary Outcome Measures

Name	Time	Method
Oocyte grading	after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started	Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes
Day-3 Embryo Grading	Three days after the intracytoplasminc sperm injection(ICSI) procedure	Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes
Day-5 Embryo Grading	5 days after the intracytoplasminc sperm injection(ICSI) procedure	Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes
beta hCG results of the transferred embryos	ten days after the embryo transfer	Clinical pregnancy outcomes

Secondary Outcome Measures

Name	Time	Method
micro RNA expression in culture medium	Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment	In the culture medium of blastocysts are going to be analyzed for microRNA levels that are expressed

Trial Locations

Locations (1)

Acibadem University Atakent Hospital; 🇹🇷 Istanbul, Turkey