Comparison of Mucin Levels at the Ocular Surface of Visual Display Users With and Without Dry Eye Disease

Completed

• Conditions: Dry Eye Disease
• Interventions: Other: no intervention

• Registration Number: NCT05604651
• Lead Sponsor: Peking University Third Hospital

Brief Summary

The study aimed to evaluate 1) if levels of the glycocalyx membrane mucins, including MUC1, MUC4, MUC16 and MUC20, are altered in conjunctival cells of visual display users With and Without Dry Eye Disease, and 2) if mucin levels correlate with dry eye clinical diagnostic data.

Detailed Description

The cross-sectional study aimed to evaluate 1) if levels of the glycocalyx membrane mucins, including MUC1, MUC4, MUC16 and MUC20, are altered in conjunctival cells of visual display users With and Without Dry Eye Disease, and 2) if mucin levels correlate with dry eye clinical diagnostic data.

Study Timeline

• Study Start: 2021-03-01
• Primary Completion: 2021-09-02
• Study Completion: 2021-09-02
• Status Verified: 2022-10
• Study First Submitted: 2022-10-29
• Study First Submitted QC: 2022-10-29
• Study First Posted: 2022-11-03
• Last Update Submitted: 2022-11-20
• Last Update Posted: 2022-11-23

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 94

Inclusion Criteria

• Participants who exposed to video display terminals for more than 1 year (at least 5 days per week and 6 hours per day) were enrolled and divided into two groups: (1) VDT users with DED and (2) VDT users without DED. DED was diagnosed according to the Tear Film and Ocular Surface Society (TFOS) DEWS II criteria: (1) Ocular Surface Disease Index (OSDI) score ≥ 13 and (2) one of these signs; fluorescein tear breakup time (TBUT) <10 sec; abnormal ocular surface staining (>5 corneal spots or > 9 conjunctival spots).

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Exclusion Criteria

• a history of contact lens wear or ocular surgery within 2 year of the study visit; a history of previous ocular disease; topical or systemic therapies other than artificial tears within two weeks before recruitment; and inflammatory ocular diseases, ocular surface diseases, glaucoma and systemic diseases with ocular involvement, such as meibomian gland dysfunction, diabetes, and peripheral neuropathy.

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
dry eye disease	no intervention	Dry eye disease was diagnosed according to the Tear Film and Ocular Surface Society (TFOS) DEWS II criteria: (1) Ocular Surface Disease Index (OSDI) score ≥ 13 and (2) one of these signs; fluorescein tear breakup time (TBUT) \<10 sec; abnormal ocular surface staining (\>5 corneal spots or \> 9 conjunctival spots).
control	no intervention	The controll group enrolled healthy participants without ocular and systemic diseases.

Primary Outcome Measures

Name	Time	Method
mucin expression	immediately after meeting the inclusion and exclusion criteria	The mRNA expression of mucin was measured by Quantitative real-time PCR, which is a laboratory technique of molecular biology to find out how much of a specific section of DNA there is in a sample.

Trial Locations

Locations (1)

Peking University Third Hospital; 🇨🇳 Beijing, China