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Hepatic IA Therapy in Stage B or Limited Stage C Hepatoma (HCC)

Early Phase 1
Recruiting
Conditions
Hepatocellular Carcinoma (HCC)
Interventions
Biological: IA therapy of HCC with CSR02-Fab-TF
Registration Number
NCT04601428
Lead Sponsor
Koo Foundation Sun Yat-Sen Cancer Center
Brief Summary

Intra-arterial (IA) therapy is generally used to treat HCC tumors that are too extensive to excise or treat with potentially curative local therapy. IA therapy takes advantage of the fact that the blood supply of HCC comes predominantly from the hepatic artery compared with the surrounding normal liver which is predominantly supplied by portal venous blood. The intent is to deprive the HCC of its blood supply, leading to the death of the tumor. Traditionally, various methods have been used to block the HCC blood supply, but improvements are needed. This study will investigate a new agent designed in the laboratory to block only tumor blood vessels, not blood vessels in the normal liver.

Detailed Description

Genetic testing was done to identify differences between HCC tumors and normal liver, and a protein, PLVAP, was shown to be present on the blood vessels of HCC but not on the blood vessels of normal liver. An antibody, CSR02, was made that recognizes PLVAP and then the Fab portion of that antibody was combined with tissue factor, a normal human protein that initiates the clotting cascade. The result is a manufactured (recombinant) protein called CSR02-Fab-TF. Preclinical studies in a mouse model showed that infusion of an equivalent mouse protein resulted in the necrosis (death) of a transplanted human HCC. The current study is designed first, to identify a safe and optimal dose of CSR02-Fab-TF in patients , and then second, to determine the response rate of HCC tumors to the IA administration of CSR02-Fab-TF.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
43
Inclusion Criteria
  • Age ≥ 18 years

  • Diagnosis of HCC by at least one of the following criteria:

    • Histological confirmation;
    • Magnetic resonance imaging (MRI) or computerized tomography (CT) consistent with liver cirrhosis AND at least one solid liver lesion > 1 cm with intense contrast uptake during the arterial phase followed by contrast washout during the venous phase regardless of alpha-fetal protein (AFP) level
  • Barcelona Clinic Liver Cancer (BCLC) Intermediate Stage B or limited Advanced Stage C (see Protocol Section 3.1). Patients with Stage C disease should have received or been offered and chosen not to receive systemic therapy

  • Inadequate response to prior liver-directed therapy (e.g., TACE, bland embolization, Y90, ablation, radiation therapy) to the same targeted area or progressive disease after prior liver-directed therapy) or to one or more systemic therapies

  • Not a candidate for curative resection, liver transplantation, or percutaneous ablation (See Protocol Appendix 3)

  • Eastern Collective Oncology Group (ECOG) performance status ≤1 (See Protocol Appendix 5)

  • Adequate laboratory parameters, including:

    • Serum total bilirubin ≤ 2x ULN
    • Alkaline phosphatase, aspartate aminotransferase (AST) and aspartate aminotransferase (ALT) < 5 x ULN;
    • Serum creatinine ≤ 1.5 mg/dL;
    • Prothrombin time (international normalized ratio; INR) ≤ 1.5;
    • Absolute neutrophil count > 1000/μL;
    • Platelet count > 75,000/μL;
    • Hgb > 8 g/dL
  • Acceptable pulmonary status, including room air O2 saturation > 90%

  • Child-Pugh A-B7 without clinically significant ascites (See Protocol Appendix 4)

  • Signed informed consent

  • All subjects must be surgically sterile, at least two years post-menopausal (if female), or agree to use adequate, effective contraception approved by the Investigator until two (2) months after receiving a final dose of CSR02-Fab-TF

Exclusion Criteria
  • Eligible for transplantation by Milan criteria (Protocol Appendix 3) or potentially eligible if successfully "down staged" by pre-transplant therapy

  • Prior organ transplantation

  • Any small molecule drug treatment for HCC (including TACE) within the previous 30 days, treatment with biological agents or any investigational therapy within the previous 60 days, or treatment with Y90 within the previous 90 days.

  • Previously treated malignancies from which the subject has not been disease-free for at least 2 years, except for adequately treated non-melanoma skin cancer, in situ cancer, or low-grade prostate or bladder cancer

  • Severe chronic obstructive or other pulmonary disease with hypoxemia that requires supplementary oxygen or clinically significant pleural effusions

  • New York Heart Association (NYHA) Class III or IV, cardiac disease, myocardial infarction within 3 months prior to therapy, unstable arrhythmia, symptomatic peripheral arterial vascular disease, or presence of an artificial or other vascular device requiring chronic anticoagulation (See Protocol Appendix 6)

  • Any of the following risks related to QT/QTc interval:

    • Baseline prolongation of QT/QTc interval (repeated interval > 480 milliseconds using Frederica's QT correction formula);
    • History of additional risk factors for Torsades de Pointes (e.g. heart failure, hypokalemia, family history of Long QT syndrome);
    • Concomitant medications that have a known risk for prolongation of the QT/QTc interval (see https://crediblemeds.org/new-drug-list/)
  • Major surgery, vascular injury, or serious illness within the previous 60 days

  • Known inherited thrombophilia (hypercoagulable state) or history of unprovoked venous or arterial thrombosis

  • Active, uncontrolled bacterial, viral, or fungal infections, requiring systemic therapy at screening. Subjects with prior HBV (positive HBSAg) must have HBV viral load < 2000 IU/mL or be receiving concurrent anti-HBV therapy to be eligible. Subjects on anti-HBV therapy must have been on treatment with a viral load maintained at < 2000 IU/mL for at least 4 weeks prior to first dose and continue on this same therapy throughout study treatment. Subjects with HCV infection are eligible if other eligibility criteria are met

  • Females who are breast-feeding

  • Allergy to iodinated contrast medium that is uncontrolled or refractory to medical therapy

  • Therapeutic anticoagulation that cannot be stopped 24-72 hours before treatment (per Section 4.5) and reinstituted no sooner than 72 hours after therapy

  • Any concomitant disease or condition that could interfere with the conduct of the study, or that would, in the opinion of the Investigator, pose an unacceptable risk to the subject in this study

  • Unwillingness or inability to comply with the study protocol for any reason

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Investigational ArmIA therapy of HCC with CSR02-Fab-TF-
Primary Outcome Measures
NameTimeMethod
Incidence and severity of adverse events from intra-arterial infusion of CSR02-Fab-TF in patients with hepatoma only or largely confined to the liver, and resistant/recurrent after prior therapyInfusion to Day 50

Measured by the number of treatment-emergent adverse events and graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) v5.0

HCC blood flowMRI on Day 4

HCC blood flow will be assessed by magnetic resonance imaging (MRI) on day 4 after infusion of CSR02-FabTF.

Secondary Outcome Measures
NameTimeMethod
Determine tumor response to intra-arterial infusion of CSR02-Fab-TFby MRI on Day 50 and then every 3 months for an average of one year.

Measured by radiographic response using acceptable imaging modalities used for assessment of tumor vasculature and blood flow (MRI or CT) based on mRECIST.

Trial Locations

Locations (4)

National Cheng Kung University Hospital (NCKUH)

🇨🇳

Tainan City, North Dist., Taiwan

National Taiwan University Hospital (NTUH)

🇨🇳

Taipei city, Zhongzheng Dist., Taiwan

Koo Foundation Sun Yat-Sen Cancer Center (KFSYSCC)

🇨🇳

Taipei, Pei-Tou Dist., Taiwan

KFSYSCC

🇨🇳

Taipei, Taiwan

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